Loading…
Optical Nanosensors for Chemical Analysis inside Single Living Cells. 2. Sensors for pH and Calcium and the Intracellular Application of PEBBLE Sensors
Optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced for intracellular measurements of pH and calcium. Five varieties of pH-sensitive sensors and three different calcium-selective sensors are presented and discussed. Each sensor combines an io...
Saved in:
Published in: | Analytical chemistry (Washington) 1999-11, Vol.71 (21), p.4837-4843 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-a471t-99a9872519d90cb74528cc2cf9b1bd23c47f8a52c9957e7d4f9cec02be9a05683 |
---|---|
cites | cdi_FETCH-LOGICAL-a471t-99a9872519d90cb74528cc2cf9b1bd23c47f8a52c9957e7d4f9cec02be9a05683 |
container_end_page | 4843 |
container_issue | 21 |
container_start_page | 4837 |
container_title | Analytical chemistry (Washington) |
container_volume | 71 |
creator | Clark, Heather A Kopelman, Raoul Tjalkens, Ron Philbert, Martin A |
description | Optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced for intracellular measurements of pH and calcium. Five varieties of pH-sensitive sensors and three different calcium-selective sensors are presented and discussed. Each sensor combines an ion-selective fluorescent indicator and an ion-insensitive internal standard entrapped within an acrylamide polymeric matrix. Calibrations and linear ranges are presented for each sensor. The photobleaching of dyes incorporated into PEBBLEs is comparable to that of the respective free dye that is incorporated within the matrix. These PEBBLE sensors are fully reversible over many measurements. The leaching of fluorescent indicator from the polymer is less than 50% over a 48-h period (note that a typical application time is only a few hours). The PEBBLE sensors have also been applied to intracellular analysis of the calcium flux in the cytoplasm of neural cells during the mitochondrial permeability transition. Specifically, a distinct difference is noted between cells of different types (astrocyte vs neuron-derived cells) with respect to their response to the toxicant m-dinitrobenzene (DNB). Use of PEBBLE sensors permits the quantitative discrimination of subtle differences between the ability of human SY5Y neuroblastoma and C6 glioma to respond to challenge with DNB. Specifically, measurement of intracellular calcium, the precursor to cell death, has been achieved. |
doi_str_mv | 10.1021/ac990630n |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_217872363</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>46534352</sourcerecordid><originalsourceid>FETCH-LOGICAL-a471t-99a9872519d90cb74528cc2cf9b1bd23c47f8a52c9957e7d4f9cec02be9a05683</originalsourceid><addsrcrecordid>eNplkc9u1DAQxi0EokvhwAsgC8GBQxbbieP4uI22tNKq3WpbgbhYE8ehLlkn2EnVPgmvi2m2f6SeZqT5zTcz3yD0npI5JYx-BS0lyVPiXqAZ5YwkeVGwl2hGCEkTJgjZQ29CuCKEUkLz12iPEp5zJvgM_T3tB6uhxSfgumBc6HzATedxeWm2d4WFg_Y22ICtC7Y2eGPdr9bglb2OCS5N24Y5ZnO8edLcH2FwNS6h1Xbc3uXDpcHHbvCgY8fYgseLvm_jhMF2DncNXi8PDlbLe5m36FUDbTDvdnEfXRwuz8ujZHX67bhcrBLIBB0SKUEWgnEqa0l0JTLOCq2ZbmRFq5qlOhNNAZxFg7gwos4aqY0mrDISogdFuo8-Trq97_6MJgzqqht9PDkoRkWUTvM0Ql8mSPsuBG8a1Xu7BX-rKFH_P6AePhDZDzvBsdqa-gk5WR6BTzsAQvS38eC0DY8clUJkMmLJhNkwmJuHMvjfKhep4Op8vVHk-8-Tw3R9pn5E_vPEgw6PNzzf7x8aHai3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217872363</pqid></control><display><type>article</type><title>Optical Nanosensors for Chemical Analysis inside Single Living Cells. 2. Sensors for pH and Calcium and the Intracellular Application of PEBBLE Sensors</title><source>American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)</source><creator>Clark, Heather A ; Kopelman, Raoul ; Tjalkens, Ron ; Philbert, Martin A</creator><creatorcontrib>Clark, Heather A ; Kopelman, Raoul ; Tjalkens, Ron ; Philbert, Martin A</creatorcontrib><description>Optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced for intracellular measurements of pH and calcium. Five varieties of pH-sensitive sensors and three different calcium-selective sensors are presented and discussed. Each sensor combines an ion-selective fluorescent indicator and an ion-insensitive internal standard entrapped within an acrylamide polymeric matrix. Calibrations and linear ranges are presented for each sensor. The photobleaching of dyes incorporated into PEBBLEs is comparable to that of the respective free dye that is incorporated within the matrix. These PEBBLE sensors are fully reversible over many measurements. The leaching of fluorescent indicator from the polymer is less than 50% over a 48-h period (note that a typical application time is only a few hours). The PEBBLE sensors have also been applied to intracellular analysis of the calcium flux in the cytoplasm of neural cells during the mitochondrial permeability transition. Specifically, a distinct difference is noted between cells of different types (astrocyte vs neuron-derived cells) with respect to their response to the toxicant m-dinitrobenzene (DNB). Use of PEBBLE sensors permits the quantitative discrimination of subtle differences between the ability of human SY5Y neuroblastoma and C6 glioma to respond to challenge with DNB. Specifically, measurement of intracellular calcium, the precursor to cell death, has been achieved.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac990630n</identifier><identifier>PMID: 10565275</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological and medical sciences ; Calcium ; Calcium - analysis ; Calibration ; Cancer ; Cell Membrane Permeability - drug effects ; Cells ; Chemistry ; Coloring Agents - chemistry ; Dinitrobenzenes - pharmacology ; Diverse techniques ; Fluoresceins - chemistry ; Fluorescent Dyes - chemistry ; Fundamental and applied biological sciences. Psychology ; Glioma - drug therapy ; Glioma - metabolism ; Humans ; Hydrogen-Ion Concentration ; Intracellular Membranes - drug effects ; Microscopy, Confocal - methods ; Mitochondria - drug effects ; Mitochondria - metabolism ; Molecular and cellular biology ; Neuroblastoma - drug therapy ; Neuroblastoma - metabolism ; Optics ; Optics and Photonics - instrumentation ; Organic Chemicals ; Photochemistry - methods ; Prostheses and Implants ; Sensors ; Tumor Cells, Cultured</subject><ispartof>Analytical chemistry (Washington), 1999-11, Vol.71 (21), p.4837-4843</ispartof><rights>Copyright © 1999 American Chemical Society</rights><rights>2000 INIST-CNRS</rights><rights>Copyright American Chemical Society Nov 1, 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a471t-99a9872519d90cb74528cc2cf9b1bd23c47f8a52c9957e7d4f9cec02be9a05683</citedby><cites>FETCH-LOGICAL-a471t-99a9872519d90cb74528cc2cf9b1bd23c47f8a52c9957e7d4f9cec02be9a05683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1197749$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10565275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clark, Heather A</creatorcontrib><creatorcontrib>Kopelman, Raoul</creatorcontrib><creatorcontrib>Tjalkens, Ron</creatorcontrib><creatorcontrib>Philbert, Martin A</creatorcontrib><title>Optical Nanosensors for Chemical Analysis inside Single Living Cells. 2. Sensors for pH and Calcium and the Intracellular Application of PEBBLE Sensors</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced for intracellular measurements of pH and calcium. Five varieties of pH-sensitive sensors and three different calcium-selective sensors are presented and discussed. Each sensor combines an ion-selective fluorescent indicator and an ion-insensitive internal standard entrapped within an acrylamide polymeric matrix. Calibrations and linear ranges are presented for each sensor. The photobleaching of dyes incorporated into PEBBLEs is comparable to that of the respective free dye that is incorporated within the matrix. These PEBBLE sensors are fully reversible over many measurements. The leaching of fluorescent indicator from the polymer is less than 50% over a 48-h period (note that a typical application time is only a few hours). The PEBBLE sensors have also been applied to intracellular analysis of the calcium flux in the cytoplasm of neural cells during the mitochondrial permeability transition. Specifically, a distinct difference is noted between cells of different types (astrocyte vs neuron-derived cells) with respect to their response to the toxicant m-dinitrobenzene (DNB). Use of PEBBLE sensors permits the quantitative discrimination of subtle differences between the ability of human SY5Y neuroblastoma and C6 glioma to respond to challenge with DNB. Specifically, measurement of intracellular calcium, the precursor to cell death, has been achieved.</description><subject>Biological and medical sciences</subject><subject>Calcium</subject><subject>Calcium - analysis</subject><subject>Calibration</subject><subject>Cancer</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cells</subject><subject>Chemistry</subject><subject>Coloring Agents - chemistry</subject><subject>Dinitrobenzenes - pharmacology</subject><subject>Diverse techniques</subject><subject>Fluoresceins - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glioma - drug therapy</subject><subject>Glioma - metabolism</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intracellular Membranes - drug effects</subject><subject>Microscopy, Confocal - methods</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Neuroblastoma - drug therapy</subject><subject>Neuroblastoma - metabolism</subject><subject>Optics</subject><subject>Optics and Photonics - instrumentation</subject><subject>Organic Chemicals</subject><subject>Photochemistry - methods</subject><subject>Prostheses and Implants</subject><subject>Sensors</subject><subject>Tumor Cells, Cultured</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNplkc9u1DAQxi0EokvhwAsgC8GBQxbbieP4uI22tNKq3WpbgbhYE8ehLlkn2EnVPgmvi2m2f6SeZqT5zTcz3yD0npI5JYx-BS0lyVPiXqAZ5YwkeVGwl2hGCEkTJgjZQ29CuCKEUkLz12iPEp5zJvgM_T3tB6uhxSfgumBc6HzATedxeWm2d4WFg_Y22ICtC7Y2eGPdr9bglb2OCS5N24Y5ZnO8edLcH2FwNS6h1Xbc3uXDpcHHbvCgY8fYgseLvm_jhMF2DncNXi8PDlbLe5m36FUDbTDvdnEfXRwuz8ujZHX67bhcrBLIBB0SKUEWgnEqa0l0JTLOCq2ZbmRFq5qlOhNNAZxFg7gwos4aqY0mrDISogdFuo8-Trq97_6MJgzqqht9PDkoRkWUTvM0Ql8mSPsuBG8a1Xu7BX-rKFH_P6AePhDZDzvBsdqa-gk5WR6BTzsAQvS38eC0DY8clUJkMmLJhNkwmJuHMvjfKhep4Op8vVHk-8-Tw3R9pn5E_vPEgw6PNzzf7x8aHai3</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>Clark, Heather A</creator><creator>Kopelman, Raoul</creator><creator>Tjalkens, Ron</creator><creator>Philbert, Martin A</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope></search><sort><creationdate>19991101</creationdate><title>Optical Nanosensors for Chemical Analysis inside Single Living Cells. 2. Sensors for pH and Calcium and the Intracellular Application of PEBBLE Sensors</title><author>Clark, Heather A ; Kopelman, Raoul ; Tjalkens, Ron ; Philbert, Martin A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a471t-99a9872519d90cb74528cc2cf9b1bd23c47f8a52c9957e7d4f9cec02be9a05683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Calcium</topic><topic>Calcium - analysis</topic><topic>Calibration</topic><topic>Cancer</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Cells</topic><topic>Chemistry</topic><topic>Coloring Agents - chemistry</topic><topic>Dinitrobenzenes - pharmacology</topic><topic>Diverse techniques</topic><topic>Fluoresceins - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glioma - drug therapy</topic><topic>Glioma - metabolism</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intracellular Membranes - drug effects</topic><topic>Microscopy, Confocal - methods</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Neuroblastoma - drug therapy</topic><topic>Neuroblastoma - metabolism</topic><topic>Optics</topic><topic>Optics and Photonics - instrumentation</topic><topic>Organic Chemicals</topic><topic>Photochemistry - methods</topic><topic>Prostheses and Implants</topic><topic>Sensors</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clark, Heather A</creatorcontrib><creatorcontrib>Kopelman, Raoul</creatorcontrib><creatorcontrib>Tjalkens, Ron</creatorcontrib><creatorcontrib>Philbert, Martin A</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clark, Heather A</au><au>Kopelman, Raoul</au><au>Tjalkens, Ron</au><au>Philbert, Martin A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optical Nanosensors for Chemical Analysis inside Single Living Cells. 2. Sensors for pH and Calcium and the Intracellular Application of PEBBLE Sensors</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>71</volume><issue>21</issue><spage>4837</spage><epage>4843</epage><pages>4837-4843</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced for intracellular measurements of pH and calcium. Five varieties of pH-sensitive sensors and three different calcium-selective sensors are presented and discussed. Each sensor combines an ion-selective fluorescent indicator and an ion-insensitive internal standard entrapped within an acrylamide polymeric matrix. Calibrations and linear ranges are presented for each sensor. The photobleaching of dyes incorporated into PEBBLEs is comparable to that of the respective free dye that is incorporated within the matrix. These PEBBLE sensors are fully reversible over many measurements. The leaching of fluorescent indicator from the polymer is less than 50% over a 48-h period (note that a typical application time is only a few hours). The PEBBLE sensors have also been applied to intracellular analysis of the calcium flux in the cytoplasm of neural cells during the mitochondrial permeability transition. Specifically, a distinct difference is noted between cells of different types (astrocyte vs neuron-derived cells) with respect to their response to the toxicant m-dinitrobenzene (DNB). Use of PEBBLE sensors permits the quantitative discrimination of subtle differences between the ability of human SY5Y neuroblastoma and C6 glioma to respond to challenge with DNB. Specifically, measurement of intracellular calcium, the precursor to cell death, has been achieved.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>10565275</pmid><doi>10.1021/ac990630n</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0003-2700 |
ispartof | Analytical chemistry (Washington), 1999-11, Vol.71 (21), p.4837-4843 |
issn | 0003-2700 1520-6882 |
language | eng |
recordid | cdi_proquest_journals_217872363 |
source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
subjects | Biological and medical sciences Calcium Calcium - analysis Calibration Cancer Cell Membrane Permeability - drug effects Cells Chemistry Coloring Agents - chemistry Dinitrobenzenes - pharmacology Diverse techniques Fluoresceins - chemistry Fluorescent Dyes - chemistry Fundamental and applied biological sciences. Psychology Glioma - drug therapy Glioma - metabolism Humans Hydrogen-Ion Concentration Intracellular Membranes - drug effects Microscopy, Confocal - methods Mitochondria - drug effects Mitochondria - metabolism Molecular and cellular biology Neuroblastoma - drug therapy Neuroblastoma - metabolism Optics Optics and Photonics - instrumentation Organic Chemicals Photochemistry - methods Prostheses and Implants Sensors Tumor Cells, Cultured |
title | Optical Nanosensors for Chemical Analysis inside Single Living Cells. 2. Sensors for pH and Calcium and the Intracellular Application of PEBBLE Sensors |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T04%3A59%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optical%20Nanosensors%20for%20Chemical%20Analysis%20inside%20Single%20Living%20Cells.%202.%20Sensors%20for%20pH%20and%20Calcium%20and%20the%20Intracellular%20Application%20of%20PEBBLE%20Sensors&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Clark,%20Heather%20A&rft.date=1999-11-01&rft.volume=71&rft.issue=21&rft.spage=4837&rft.epage=4843&rft.pages=4837-4843&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac990630n&rft_dat=%3Cproquest_cross%3E46534352%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a471t-99a9872519d90cb74528cc2cf9b1bd23c47f8a52c9957e7d4f9cec02be9a05683%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=217872363&rft_id=info:pmid/10565275&rfr_iscdi=true |