Feasibility of High-Performance Immunochromatography as an Isolation Method for PCBs and Other Dioxin-Like Compounds

A high performance immunochromatographic procedure to isolate polychlorinated biphenyls (PCBs) and other dioxin-like compounds from a sample is shown. Development of the procedure includes (i) synthesis of the hapten, binding it to the spacer arm and to the carrier protein to make the immunizing mol...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-07, Vol.73 (13), p.3119-3125
Main Authors: Concejero, Miguel Angel, Galve, Roger, Herradón, Bernardo, González, Maria Jose, de Frutos, Mercedes
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Biotechnology
Chromatography
Environment and pollution
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Global environmental pollution
Immunology
Industrial applications and implications. Economical aspects
Miscellaneous
PCB
Pollution
Polychlorinated biphenyls
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Summary:A high performance immunochromatographic procedure to isolate polychlorinated biphenyls (PCBs) and other dioxin-like compounds from a sample is shown. Development of the procedure includes (i) synthesis of the hapten, binding it to the spacer arm and to the carrier protein to make the immunizing molecule; (ii) raising and purification of anti-PCB antibodies; (iii) building of the immunocolumn; (iv) selection of the binding, rinsing, and elution conditions adequate for these highly lipophilic compounds; (v) study of the influence of the concentration and volume of sample on recovery; and (vi) study of the selectivity of the immunosystem for dioxins, furans, PCBs, and several insecticides of different toxicity. Evaluation of the method is carried out by analyzing the fractions retained and nonretained in the immunocolumn by GC/MS. The immunochromatographic system that is developed shows itself to be feasible as cleanup and isolation steps carried out prior to GC/MS analyses. When compared to classical cleanup and isolation methods traditionally used for analysis of PCBs in water, the immunochromatographic method is >20× faster and uses 100× less organic solvents, and its selectivity is enormously enhanced. Good recoveries are obtained with both kinds of methods. The immunochromatographic procedure fulfils the acceptance criteria indicated by the EPA, even for sub-parts-per-billion concentrations.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac001387r