Mechanistic Studies of Partial-Filling Micellar Electrokinetic Chromatography

The need for coupling micellar electrokinetic chromatography (MEKC) with electrospray mass spectrometry initiates the development of partial-filling MEKC. In comparison with conventional MEKC, only a small portion of the capillary is filled with a micellar solution for performing the separation in p...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1996-09, Vol.68 (18), p.3265-3269
Main Authors: Nelson, Wendy M, Lee, Cheng S
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography - methods
Electrochemistry
Exact sciences and technology
Herbicides
Herbicides - analysis
Kinetics
Micelles
Other chromatographic methods
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Summary:The need for coupling micellar electrokinetic chromatography (MEKC) with electrospray mass spectrometry initiates the development of partial-filling MEKC. In comparison with conventional MEKC, only a small portion of the capillary is filled with a micellar solution for performing the separation in partial-filling MEKC. Analytes first migrate into the micellar plug, where the separation occurs, and then into the leading electrophoresis buffer, which is free of surfactants. A theoretical model is proposed for predicting the separation behavior of triazine herbicides in partial-filling MEKC. The comparisons between conventional and partial-filling MEKC in terms of separation efficiency and resolution of triazine herbicides are presented and discussed. The optimization techniques, possible applications, and advantages of partial-filling MEKC are similarly addressed.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac951137o