Monitoring Polycyclic Aromatic Hydrocarbon Metabolites in Human Urine:  Extraction and Purification with a Sol−Gel Glass Immunosorbent

A new, rapid method for selective extraction of hydroxylated polycyclic aromatic hydrocarbons metabolites (OH-PAHs) in human urine was developed using an immunosorbent of anti-pyrene antibodies which were encapsulated in a sol−gel glass (SGG) matrix. Resulting chromatograms after immunoextraction of...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-12, Vol.73 (23), p.5669-5676
Main Authors: Schedl, Matthias, Wilharm, Gottfried, Achatz, Stefan, Kettrup, Antonius, Niessner, Reinhard, Knopp, Dietmar
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Carcinogens - analysis
Chemical and industrial products toxicology. Toxic occupational diseases
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid
Exact sciences and technology
Gas Chromatography-Mass Spectrometry
Humans
Hydrocarbons
Immunologic Techniques
Medical sciences
Other chromatographic methods
Polycyclic Aromatic Hydrocarbons - urine
Smoking - urine
Spectrophotometry, Ultraviolet
Toxicology
Urine
Various organic compounds
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Summary:A new, rapid method for selective extraction of hydroxylated polycyclic aromatic hydrocarbons metabolites (OH-PAHs) in human urine was developed using an immunosorbent of anti-pyrene antibodies which were encapsulated in a sol−gel glass (SGG) matrix. Resulting chromatograms after immunoextraction of urine samples and HPLC analysis of the extracts were free from matrix interferences. The LODs for the determination of OH-PAHs in these difficult samples were in the low-ppt range (1−16 ng/L). In addition to its high selectivity, the immunosorbent proved to be robust and reusable. Obtained recoveries in spiked urine samples ranged from 83 to 107% for the hydroxyphenanthrene and hydroxypyrene compounds under investigation, while recovery for 3-hydroxybenzo[a]pyrene was only 45−62%. In a biomonitoring study, the SGG immunosorbent was successfully used for trace-level analysis of OH-PAHs in 20 human urine samples. Results were compared to data obtained by an independent reference analysis method and revealed good correlation between both methods.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac010868n