Characteristics of Trapping Various Organophosphorus Pesticides with a Ferritin Reactor of Shark Liver (Sphyrna z ygaena)

A reactor is composed of liver ferritin of Sphyrna zygaena (SZLF) and an oscillating bag. A reactive procedure for trapping various organphosphorus pesticides (OPs) with the SZLF reactor in the flowing water is described in detail, showing the maximal trapping numbers of 28 ± 1.0 dichlorovos/SZLF, 4...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2005-03, Vol.77 (6), p.1920-1927
Main Authors: Huang, He-Qing, Xiao, Zhi-Qun, Lin, Qing-Mei, Chen, Ping
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Chemistry
Dichlorvos - analysis
Diffusion
Exact sciences and technology
Ferritins - chemistry
Hydrogen-Ion Concentration
Liver
Liver - chemistry
Organophosphorus Compounds - analysis
Organothiophosphorus Compounds - analysis
Pesticides
Pesticides - analysis
Scientific apparatus & instruments
Sharks - metabolism
Spectrometric and optical methods
Water filtration
Water Pollutants - analysis
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Summary:A reactor is composed of liver ferritin of Sphyrna zygaena (SZLF) and an oscillating bag. A reactive procedure for trapping various organphosphorus pesticides (OPs) with the SZLF reactor in the flowing water is described in detail, showing the maximal trapping numbers of 28 ± 1.0 dichlorovos/SZLF, 42 ± 1.0 dimethoate/SZLF, and 55 ± 1.0 methamido-phos/SZLF determined by a improved spectrophotometric method in 12 h. In addition, it is found that the OP numbers trapped by the reactor increase along with the incubation time and its concentration increment in the flowing water (or seawater), respectively. This trapping capacity is considered to depend on the composition of amino acids on the surface of the ferritin shell interior rather than the available volume within the shell. A novel pathway for trapping various OPs with the ferritin is suggested in reference to unstable characteristics of the protein subunits. We claim that the ferritin reactor will be employed to monitor the contamination level of various OPs in the flowing water continuously.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac048753h