Dynamic Cross-Linking Effect of Mg^sup 2+^ To Enhance Sieving Properties of Low-Viscosity Poly(vinylpyrrolidone) Solutions for Microchip Electrophoresis of Proteins

We report a dynamic cross-linking effect of Mg... that enhances the sieving properties of low-viscosity poly(vinylpyrrolidone) (PVP) solutions. A low-viscosity PVP solution was applied to nondenaturing microchip electrophoresis of protein samples using microchips made of poly(methyl methacrylate). T...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2008-01, Vol.80 (1), p.312
Main Authors: Mohamadi, Mohamad Reza, Kaji, Noritada, Tokeshi, Manabu, Baba, Yoshinobu
Format: Article
Language:English
Subjects:
Analytical chemistry
Aqueous solutions
Correlation analysis
Polymers
Proteins
Studies
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Summary:We report a dynamic cross-linking effect of Mg... that enhances the sieving properties of low-viscosity poly(vinylpyrrolidone) (PVP) solutions. A low-viscosity PVP solution was applied to nondenaturing microchip electrophoresis of protein samples using microchips made of poly(methyl methacrylate). The separation resolution of nondenatured protein markers in 1.8% PVP solution was improved by adding 1-20 mM MgCl... We studied the effect of the ratio of cross-linking agent on mobility of protein samples and showed that protein retardation (...) is correlated with the ratio of cross-linking agent to PVP (...) as ln ... = ... A' was related to the protein radius (R), and b' was found to be 0.72 for proteins with R = 2.4 nm and 0.82 for proteins with R = 1.85 nm. A structural study of PVP in semidilute solutions using dynamic light scattering showed that incremental increases of Mg... ion concentration from 5 to 20 mM in 1.8% PVP solution increased the hydrodynamic radius of PVP polymers by 20%. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0003-2700
1520-6882