Pedobacter pollutisoli sp. nov., Isolated from Tetrabromobisphenol A-Contaminated Soil

• Published in: Current microbiology 2019-04, Vol.76 (4), p.442-447
• Main Authors: Mu, Yang, Ke, Zhuang, Feng, Chen-Xi, Wang, Xiao-Wei, Wang, Xiao-Wen, Wang, Hong-Mei, Chen, Qing, He, Jian
• Format: Article
• Language: English
• Subjects: Base Composition; Biomedical and Life Sciences; Biotechnology; China; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - chemistry; Gene sequencing; Life Sciences; Lipids; Microbiology; New species; Nucleic Acid Hybridization; Pedobacter - chemistry; Pedobacter - classification; Pedobacter - isolation & purification; Pedobacter - physiology; Phosphatidylethanolamine; Phospholipids; Phospholipids - chemistry; Phylogenetics; Phylogeny; Polybrominated Biphenyls - chemistry; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Soil - chemistry; Soil analysis; Soil contamination; Soil Microbiology; Soil Pollutants - chemistry; Soil pollution; Species Specificity; Tetrabromobisphenol A; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-019-01643-6

A Gram-stain negative, strictly aerobic, non-spore forming, non-motile, rod-shaped bacterium, designated TBBPA-24 T , was isolated from tetrabromobisphenol A-contaminated soil in China. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain TBBPA-24 T was most closely related to Pedobacter nanyangensis Q4 T (96.5%) and Pedobacter ‘zeaxanthinifaciens’ TDMA-5 T (96.1%). Chemotaxonomic analysis revealed that strain TBBPA-24 T possessed MK-7 as the major respiratory quinone and lipid, aminolipid, phospholipid, phosphatidylethanolamine, and phosphoaminolipid as the major polar lipid. The major fatty acids were iso-C 15:0 (40.2%), summed feature 3 (C 16:1 ω 6 c and/or C 16:1 ω 7 c , 25.6%) and iso-C 17:0 3-OH (16.4%). The genomic DNA G+C content of strain TBBPA-24 T was 43.9 mol%. Based on the phylogenetic, phenotypic characteristics, and chemotaxonomic data, strain TBBPA-24 T is considered a novel species of the genus Pedobacter , for which the name Pedobacter pollutisoli sp. nov. is proposed. The type strain TBBPA-24 T (= KCTC 62314 T  = CCTCC AB 2017244 T ) is proposed.