Effects of gossypol from cottonseed meal and dietary vitamin E on the reproductive characteristics of superovulated beef heifers

• Published in: Journal of animal science 2002-09, Vol.80 (9), p.2485-2492
• Main Authors: Velasquez-Pereira, J, Arechiga, C. F, McDowell, L. R, Hansen, P. J, Chenoweth, P. J, Calhoun, M. C, Risco, C. A, Batra, T. R, Williams, S. N, Wilkinson, N. S
• Format: Article
• Language: English
• Subjects: alpha-Tocopherol - blood; Animal Feed; Animal productions; Animal reproduction; Animals; Biological and medical sciences; Cattle; Cattle - blood; Cattle - embryology; Cattle - physiology; Cottonseed Oil; Diet; Dose-Response Relationship, Drug; Embryo, Mammalian - metabolism; Endometrium - metabolism; Endometrium - physiology; Erythrocytes - metabolism; Female; Fundamental and applied biological sciences. Psychology; Gossypol - administration & dosage; Gossypol - adverse effects; Isomerism; Nutrition; Osmotic Fragility; Pregnancy; Random Allocation; Superovulation; Terrestrial animal productions; Vertebrates; Vitamin E; Vitamin E - administration & dosage
• ISSN: 0021-8812; 1525-3163; 1525-3163
• DOI: 10.2527/2002.8092485x

Superovulated Hereford-Angus crossbred heifers (average 397 kg BW) were used to test the effect of feeding cottonseed meal (gossypol) and vitamin E on embryo quality and ovarian characteristics. Twenty-four heifers were assigned randomly to four treatments with six heifers per treatment. Treatments were the following dietary supplements: 1) SBM (soybean meal + 30 IU vitamin E/kg of diet DM); 2) SBM+E (soybean meal + 4,000 IU vitamin E x animal(-1) x d(-1)); 3) CSM (cottonseed meal + 30 IU vitamin E/kg of diet DM); and 4) CSM+E (cottonseed meal + 4,000 IU vitamin E x animal(-1) x d(-1)). Supplements based on cottonseed meal provided 43.5 g of total gossypol/d (37% negative isomer (-) and 63% positive isomer (+)). Blood samples were collected at the start of the experiment and every 3 wk thereafter up to 12 wk. Plasma a-tocopherol (alpha-T) concentration was affected by treatments (P < 0.05). Heifers supplemented with cottonseed meal had greater (P < 0.05) alpha-T concentration in plasma than heifers supplemented with soybean meal at each concentration of vitamin E. Supplementation at 4,000 IU vitamin E x animal(-1) d(-1) increased (P < 0.05) the concentration of a-T in plasma. Weight gain, hemoglobin and hematocrit were not affected by treatment. Erythrocyte osmotic fragility (EOF) increased (P < 0.05) in cottonseed meal-fed animals; however, EOF was lowered (P < 0.05) with vitamin E supplementation. Heifers fed CSM and CSM+E supplements had greater (P < 0.01) concentrations of (-)-, (+)-, and total-gossypol in plasma, corpora lutea (CL), liver, and endometrium than heifers fed SBM and SBM+E supplements. Tissue alpha-T concentration increased with increased dietary supplemental vitamin E, particularly in great amounts in the CL. Because there was no adverse effect of gossypol on superovulation response or embryo development despite concentrations of gossypol in endometrium that are toxic to embryos, it is likely that systems exist in the reproductive tract to limit gossypol toxicity.