Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock

The study aimed at the production and characterization of biopolymer polyhydroxyalkanoate (PHA) by Bacillus endophyticus . The usage of refined (sucrose 2–4%) and inexpensive unrefined (sugar cane molasses 2–4%) carbon source was evaluated by varying the media components via shake flask method and b...

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Bibliographic Details
Published in:Journal of polymers and the environment 2019-05, Vol.27 (5), p.917-928
Main Authors: Geethu, M., Vrundha, R., Raja, S., Raghu Chandrashekar, H., Divyashree, M. S.
Format: Article
Language:English
Subjects:
Bacillus endophyticus
Biodegradable materials
Biopolymers
Bioreactors
Carbon
Carbon sources
Cell density
Chemistry
Chemistry and Materials Science
Cultivation
Culture media
Density
Design of experiments
Endophytes
Environmental Chemistry
Environmental Engineering/Biotechnology
Experimental design
Industrial Chemistry/Chemical Engineering
Materials Science
Mathematical models
Molasses
NMR
Nuclear magnetic resonance
Nutrients
Original Paper
Polyhydroxyalkanoates
Polyhydroxyalkanoic acid
Polymer Sciences
Statistical models
Sucrose
Sugar
Sugarcane
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Summary:The study aimed at the production and characterization of biopolymer polyhydroxyalkanoate (PHA) by Bacillus endophyticus . The usage of refined (sucrose 2–4%) and inexpensive unrefined (sugar cane molasses 2–4%) carbon source was evaluated by varying the media components via shake flask method and batch cultivation in bioreactor. The optimal PHA production of 10.7 g/L of PHA from 15.37 g/L of cell density was obtained using 4% sugarcane molasses (SCM) at 250 rpm using optimized medium obtained through statistical experimental design. Batch cultivation with increased agitation rate and addition of feeding nutrients provided by SCM significantly lead to enhanced PHA production by the test organism by increasing the overall cell density. According to the statistical model, the increase in the sugar concentration (> 4%) by SCM had a negative effect on PHA production. Characterization by FTIR and 1 H NMR was performed to confirm the biopolymer that has been produced by Bacillus spp.
ISSN:1566-2543
1572-8919
DOI:10.1007/s10924-019-01397-z