Developmental expression of three prmt genes in Xenopus

Protein arginine methyltransferases (PRMTs) are involved in many cellular processes via the arginine methylation of histone or non-histone proteins. We examined the expression patterns of prmt4, prmt7, and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative...

Full description

Saved in:
Bibliographic Details
Published in:Dōngwùxué yánjiū 2019-03, Vol.40 (2), p.102-107
Main Authors: Wang, Cheng-Dong, Guo, Xiao-Fang, Wong, Thomas Chi Bun, Wang, Hui, Qi, Xu-Feng, Cai, Dong-Qing, Deng, Yi, Zhao, Hui
Format: Article
Language:English
Subjects:
Animal embryos
Arginine
Developmental stages
DNA
Embryogenesis
Embryology
Embryonic growth stage
Embryos
Frogs
Gene expression
Genes
Histones
Hybridization
Methylation
Nucleotide sequence
PCR
Polymerase chain reaction
Proteins
Reverse transcription
Transcription
Xenopus
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Protein arginine methyltransferases (PRMTs) are involved in many cellular processes via the arginine methylation of histone or non-histone proteins. We examined the expression patterns of prmt4, prmt7, and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative reverse transcription polymerase chain reaction (RT-PCR). Specific prmt9 signals were not detected in Xenopus embryos until the late tailbud stage when weak expression was observed in the branchial arches. Quantitative RT-PCR indicated that the expression of prmt4 and prmt7 was up-regulated during the neurula stage, whereas prmt9 maintained its low expression until the late tailbud stage, consistent with the whole-mount in situ hybridization results. Thus, the developmental expression patterns of these three prmt genes in Xenopus embryos provide a basis for further functional study of such genes.
ISSN:0254-5853
DOI:10.24272/j.issn.2095-8137.2018.064