Genome‐wide markers untangle the green‐lizard radiation in the Aegean Sea and support a rare biogeographical pattern

• Published in: Journal of biogeography 2019-03, Vol.46 (3), p.552-567
• Main Authors: Kornilios, Panagiotis, Thanou, Evanthia, Lymberakis, Petros, Ilgaz, Çetin, Kumlutaş, Yusuf, Leaché, Adam
• Format: Article
• Language: English
• Subjects: Aegean Sea barrier; Anatolia; Barriers; Cytochrome; Cytochrome b; Cytochromes; ddRAD; Deoxyribonucleic acid; Dispersal; Dispersion; DNA; east Mediterranean; genome wide SNPs; Genomes; Geographical distribution; Hypotheses; Islands; Lacertidae; Lizards; Mid‐Aegean Trench; Mitochondrial DNA; overseas dispersal; phylogeography; Pliocene; Population structure; Populations; Radiation; SNAPP coalescence; Structural hierarchy; Taxa; Trees
• ISSN: 0305-0270; 1365-2699
• DOI: 10.1111/jbi.13524

Aim The Aegean Sea constitutes a major biogeographic barrier between the European and Asian continents and several models of diversification in the Aegean have been documented. Here, we test three of those models for the Aegean green‐lizards (Lacerta trilineata–pamphylica group): Vicariance versus Overland Dispersal versus Island Stepping‐stone Dispersal. We investigate these hypotheses and complement our knowledge on the impact of the Aegean Barrier on east Mediterranean taxa. Location Aegean Sea, east Mediterranean. Taxon Lacerta lizards. Methods We analysed ddRAD loci (double‐digest restriction‐site‐associated DNA) to estimate species‐trees under coalescent models and maximum likelihood trees using concatenation. We performed hierarchical population structure analyses and inferred ancestral distribution‐areas. We also sequenced the complete cytochrome b gene and produced a time‐calibrated mtDNA gene‐tree to conduct a critical comparison with previous studies. Results Aegean green‐lizards diverged into four main groups in parallel during the Late Pliocene with distributions to the East and West of the Aegean. The Eastern group includes Lacerta pamphylica and East Aegean L. trilineata, while the Western group contains the Central Cyclades populations and the remaining populations of the Balkan Peninsula. The Aegean green‐lizards’ ancestor occurred in Anatolia, while the West lineage ancestor occurred in the Central Cyclades islands, revealing a dispersal between the two regions. The radiations of all major green‐lizard groups, including trilineata+pamphylica, occurred in parallel in the Late Pliocene. Main Conclusions In contrast to previously suggested biogeographical hypotheses for the group, based on mtDNA, the Island Stepping‐stone Dispersal scenario is strongly supported. Green lizards offer a rare paradigm of diversification in the Aegean, where populations largely expanded their geographical distribution and crossed the Aegean Barrier using the central Aegean islands as stepping stones.