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Distinguishable staining with neutral red for GFP-marked and GFP-nonmarked Fusarium oxysporum strains simultaneously colonizing root surfaces
Green fluorescent protein (GFP)-marked Fusarium oxysporum f. sp. melonis and nonmarked F. oxysporum f. sp. fragariae were stained with neutral red. The neutral red stained vacuoles of the fungi without disturbing GFP fluorescence in the cytoplasm. GFP-marked fungi showed fluorescent hyphae with dark...
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Published in: | Journal of general plant pathology : JGPP 2003-02, Vol.69 (1), p.45-48 |
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creator | Nonomura, T. (Kinki Univ., Higashiosaka, Osaka (Japan). Faculty of Agriculture) Tajima, H Kitagawa, Y Sekiya, N Shitomi, K Tanaka, M Maeda, K Matsuda, Y Toyoda, H |
description | Green fluorescent protein (GFP)-marked Fusarium oxysporum f. sp. melonis and nonmarked F. oxysporum f. sp. fragariae were stained with neutral red. The neutral red stained vacuoles of the fungi without disturbing GFP fluorescence in the cytoplasm. GFP-marked fungi showed fluorescent hyphae with dark-stained vacuoles, whereas nonmarked fungi were detected as nonfluorescent hyphae with dark-dotted vacuoles. Root colonization by these two fungi was monitored using this method. Microconidia attached similarly to the root surface and elongated vegetative hyphae. Only the pathogenic fungi invaded, causing necrosis at the inoculation site. Thus, the present method enabled us to track simultaneously the various formae speciales of F. oxysporum colonizing the root surface. [PUBLICATION ABSTRACT] |
doi_str_mv | 10.1007/s10327-002-0018-7 |
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(Kinki Univ., Higashiosaka, Osaka (Japan). Faculty of Agriculture) ; Tajima, H ; Kitagawa, Y ; Sekiya, N ; Shitomi, K ; Tanaka, M ; Maeda, K ; Matsuda, Y ; Toyoda, H</creator><creatorcontrib>Nonomura, T. (Kinki Univ., Higashiosaka, Osaka (Japan). Faculty of Agriculture) ; Tajima, H ; Kitagawa, Y ; Sekiya, N ; Shitomi, K ; Tanaka, M ; Maeda, K ; Matsuda, Y ; Toyoda, H</creatorcontrib><description>Green fluorescent protein (GFP)-marked Fusarium oxysporum f. sp. melonis and nonmarked F. oxysporum f. sp. fragariae were stained with neutral red. The neutral red stained vacuoles of the fungi without disturbing GFP fluorescence in the cytoplasm. GFP-marked fungi showed fluorescent hyphae with dark-stained vacuoles, whereas nonmarked fungi were detected as nonfluorescent hyphae with dark-dotted vacuoles. Root colonization by these two fungi was monitored using this method. Microconidia attached similarly to the root surface and elongated vegetative hyphae. 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subjects | Crop diseases CUCUMIS MELO Fungi FUSARIUM OXYSPORUM GENETIC MARKERS GENETIC TRANSFORMATION PATHOGENS Plant pathology Proteins |
title | Distinguishable staining with neutral red for GFP-marked and GFP-nonmarked Fusarium oxysporum strains simultaneously colonizing root surfaces |
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