A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells

In this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 n...

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Bibliographic Details
Published in:Natural product research 2018-12, Vol.32 (24), p.2940-2944
Main Authors: Pontual, Emmanuel V., Pires-Neto, Divar F., Fraige, Karina, Higino, Taciana M. M., Carvalho, Belany E. A., Alves, Neyla M. P., Lima, Thâmarah A., Zingali, Russolina B., Coelho, Luana C. B. B., Bolzani, Vanderlan S., Figueiredo, Regina C. B. Q., Napoleão, Thiago H., Paiva, Patrícia M. G.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cercopithecus aethiops
chagas disease
Cytotoxicity
Flavonoids
Flavonoids - analysis
Flowers - chemistry
High-performance liquid chromatography
Liquid chromatography
Lysis
Macrophages
Macrophages, Peritoneal - drug effects
Mammalian cells
Mammals
Mice
Moringa oleifera
Moringa oleifera - chemistry
Peptides
Peritoneum
Plant Extracts - chemistry
Plant Extracts - pharmacology
protease inhibitor
Protozoa
Selectivity
Toxicity
Trypanocidal Agents - pharmacology
Trypanosoma cruzi
Trypanosoma cruzi - drug effects
trypomastigote
Trypomastigotes
Trypsin
Trypsin inhibitors
Trypsin Inhibitors - isolation & purification
Trypsin Inhibitors - pharmacology
Vero cells
Vero Cells - drug effects
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Summary:In this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC 50/24 h of 54.18 ± 6.62 and 41.20 ± 4.28 μg/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2017.1389932