Functional characterization of citrus polygalacturonase-inhibiting protein

A cDNA encoding a polygalacturonase-inhibiting protein gene (SaiPGIPA) was identified from the citrus cultivar Sainumphung (Citrus sp.), one of the most popular cultivars in northern Thailand. SaiPGIPA was expressed in Escherichia coli cells, and the functional properties of citrus PGIP were analyze...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2002-05, Vol.68 (2), p.118-127
Main Authors: Nalumpang, S. (Kagawa Univ., Takamatsu (Japan).), Gotoh, Y, Tsuboi, H, Gomi, K, Yamamoto, H, Akimitsu, K
Format: Article
Language:English
Subjects:
CITRUS
Citrus fruits
Cultivars
DISEASE RESISTANCE
E coli
ENZYME INHIBITORS
Fruits
GENE EXPRESSION
INDUCED RESISTANCE
Plant pathology
PLANT PROTEIN
POLYGALACTURONASE
Proteins
THAILAND
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Summary:A cDNA encoding a polygalacturonase-inhibiting protein gene (SaiPGIPA) was identified from the citrus cultivar Sainumphung (Citrus sp.), one of the most popular cultivars in northern Thailand. SaiPGIPA was expressed in Escherichia coli cells, and the functional properties of citrus PGIP were analyzed. The PGIP fusion protein inhibited by a maximum of about 60% of the endopolygalacturonase activity, and a mixture of the PGIP and fungal endopolygalacturonase released oligogalacturonides from polygalacturonic acid. The mixture containing the oligogalacturonides, endopolygalacturonase and PGIP induced expression of the PGIP gene and a chalcone synthase gene in citrus leaves. The mixture also induced resistance in cucumber leaves against Colletotrichum lagenarium
ISSN:1345-2630
1610-739X
DOI:10.1007/pl00013064