Glutathione transferase modulates acute ethanol‐induced sedation in Drosophila neurones

Heavy alcohol consumption leads to neuropathological damage and alcohol use disorder, which affects the health of people and results in a cost burden. However, the genes modulating sensitivity to ethanol remain largely unknown. Here, we identified a novel gene, Drosophila glutathione transferase ome...

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Bibliographic Details
Published in:Insect molecular biology 2019-04, Vol.28 (2), p.246-252
Main Authors: Choi, H.‐J., Cha, S. J., Kim, K.
Format: Article
Language:English
Subjects:
alcohol
Alcohols
Animals
Central Nervous System Depressants - toxicity
Dopamine receptors
Drosophila
Drosophila - drug effects
Drosophila - enzymology
Drosophila - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Ethanol
Ethanol - toxicity
ethanol sedation
Gene expression
Glutathione
Glutathione transferase
Glutathione Transferase - genetics
Glutathione Transferase - metabolism
glutathione transferase omega
Insects
Male
neurone
Neurons - drug effects
Ribonucleic acid
RNA
RNA-mediated interference
Sensitivity
Serotonin
Toxicity
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Summary:Heavy alcohol consumption leads to neuropathological damage and alcohol use disorder, which affects the health of people and results in a cost burden. However, the genes modulating sensitivity to ethanol remain largely unknown. Here, we identified a novel gene, Drosophila glutathione transferase omega 1 (GstO1), which plays a critical role in regulating sensitivity to ethanol sedation. GstO1 mutant flies showed highly increased ethanol sensitivity. Furthermore, the expression level of GstO1 regulates the behavioural response to ethanol, because decreasing and increasing GstO1 affects sedation sensitivity in a contrasting manner. In addition, the RNA interference‐mediated knockdown of GstO1 expression reveals that GstO1 mediates sensitivity to ethanol sedation in neurones, including dopaminergic and serotonergic neurones. Altogether, our findings provide the first evidence for the involvement of glutathione transferase in the response to alcohol in Drosophila and provide a novel mechanistic insight into the toxicity and sensitivity of ethanol exposure.
ISSN:0962-1075
1365-2583
DOI:10.1111/imb.12547