A molecular phylogeny of the tribe Ochthebiini (Coleoptera, Hydraenidae, Ochthebiinae)

• Published in: Systematic entomology 2019-04, Vol.44 (2), p.273-288
• Main Authors: Villastrigo, Adrián, Jäch, Manfred A., Cardoso, Anabela, Valladares, Luis F., Ribera, Ignacio
• Format: Article
• Language: English
• Subjects: Bayesian analysis; Cytochrome-c oxidase; Genera; Hydraenidae; Leucine; Mitochondria; NADH; NADH dehydrogenase; Ochthebiinae; Ochthebius; Phylogeny; Ribonucleic acid; RNA; Species; tRNA Leu
• ISSN: 0307-6970; 1365-3113
• DOI: 10.1111/syen.12318

Ochthebiinae, with c . 650 species distributed worldwide, are the second most speciose subfamily of the aquatic beetle family Hydraenidae. They are ecologically the most diverse hydraenid subfamily, with terrestrial species as well as species in almost all types of aquatic habitats, including hypersaline waters. Ochthebiinae include the tribes Ochtheosini (four species in three genera) and Ochthebiini. We provide here the first comprehensive phylogeny of the tribe Ochthebiini, based on 186 species and four subspecies from most genera, subgenera and species groups. We obtained sequence data for a combination of mitochondrial and nuclear gene fragments including the 5′ and 3′ ends of the cytochrome c oxidase subunit 1, the 5′ end of 16S RNA plus the leucine tRNA transfer plus 5′ end of NADH dehydrogenase subunit I, and internal fragments of the large and small ribosomal units. The analyses with maximum likelihood (ML) and Bayesian probabilities consistently recovered a generally well supported phylogeny, with most currently accepted taxa and species groups as monophyletic. We provide a new classification of the tribe based on our phylogenetic results, with six genera: Meropathus Enderlein, Ochthebius Leach, Protochthebius Perkins, Prototympanogaster Perkins, Tympallopatrum Perkins and Tympanogaster Janssens. The genus Ochthebius is here divided into nine subgenera in addition to Ochthebius s.s.: (1) O . ( Angiochthebius ) Jäch & Ribera; (2) O . ( Asiobates ) Thomson; (3) O . ( Aulacochthebius ) Kuwert; (4) O . ( Cobalius ) Rey; (5) O . ( Enicocerus ) Stephens; (6) O . ( Gymnanthelius ) Perkins comb.n. ; (7) O . ( Gymnochthebius ) Orchymont; (8) O . ( Hughleechia ) Perkins comb.n. ; and (9) O . ( Micragasma ) Sahlberg. Within Ochthebius s.s., 17 species groups are proposed, five of them newly established (3, 9, 11, 13 and 16): (1) andraei ; (2) atriceps ; (3) corrugatus ; (4) foveolatus ; (5) kosiensis ; (6) lobicollis ; (7) marinus ; (8) metallescens ; (9) nitidipennis ; (10) notabilis ; (11) peisonis ; (12) punctatus ; (13) quadricollis ; (14) rivalis ; (15) strigosus ; (16) sumatrensis ; and (17) vandykei . We elevated to species rank two subspecies of Ochthebius : O. fallaciosus Ganglbauer stat.n. (former subspecies of O. viridis Peyron) and O. deletus Rey stat.rest. (former subspecies of O. subpictus Wollaston).