The ICE-like transcription factor HbICE2 is involved in jasmonate-regulated cold tolerance in the rubber tree (Hevea brasiliensis)

• Published in: Plant cell reports 2019-06, Vol.38 (6), p.699-714
• Main Authors: Chen, Wei-Jie, Wang, Xue, Yan, Sa, Huang, Xi, Yuan, Hong-Mei
• Format: Article
• Language: English
• Subjects: Arabidopsis; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cold; Cold tolerance; Complementation; Cyclopentanes - pharmacology; Fluorescence; Gene expression; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - genetics; Hevea - drug effects; Hevea - genetics; Hevea - metabolism; Hevea brasiliensis; Life Sciences; Low temperature; Methyl jasmonate; Myc protein; N-Terminus; Original Article; Oxylipins - pharmacology; Plant Biochemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Proteins; Repressors; Rubber; Rubber trees; Signal transduction; Signaling; Transcription activation; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Yeast; Yeasts
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-019-02398-x

Key message An ICE-like transcription factor mediates jasmonate-regulated cold tolerance in the rubber tree ( Hevea brasiliensis ), and confers cold tolerance in transgenic Arabidopsis . The rubber tree ( Hevea brasiliensis ) is susceptible to low temperatures, and understanding the mechanisms regulating cold stress is of great potential value for enhancing tolerance to this environmental variable. In this study, we find that treatment with exogenous methyl jasmonate (MeJA) could significantly enhance Hevea brasiliensis cold tolerance. In addition, yeast two-hybrid and bimolecular fluorescence complementation (BiFC) experiments show that JASMONATE ZIM-DOMAIN(JAZ) proteins, HbJAZ1 and HbJAZ12, key repressors of JA signaling pathway, interact with HbICE2, a novel ICE (Inducer of CBF Expression)-like protein. HbICE2 was nuclear-localised and bound to the MYC recognition (MYCR) sequence. The transcriptional activation activity of HbICE2 in yeast cells was dependent on the N-terminus, and overexpression of HbICE2 in Arabidopsis resulted in elevated tolerance to chilling stress. Furthermore, dual-luciferase transient assay reveals that HbJAZ1 and HbJAZ12 proteins inhibit the transcriptional function of HbICE2. The expression of C-repeat-binding factor (CBF) signalling pathway genes including HbCBF1, HbCBF2 and HbCOR47 were up-regulated by MeJA. Taken together, our data suggest that the new ICE-like transcription factor HbICE2 is involved in jasmonate-regulated cold tolerance in Hevea brasiliensis .