Oxidative Coupling: A Tranquil Approach for Determination of Selexipag by Visible Spectrophotometry

The present study is a first report on development of a spectrophotometric method for determination of selexipag (used to cure pulmonary arterial hypertension) in bulk and tablet formulation and its validation. The basis of the proposed method is formation of a chromophore (of λ max 600 nm) in prese...

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Bibliographic Details
Published in:Oriental journal of chemistry 2018-01, Vol.34 (6), p.3112-3117
Main Authors: Gorumutchu, Giri Prasad, Ratnakaram, Venkata Nadh
Format: Article
Language:English
Subjects:
Acidic oxides
Calibration
Chromophores
Coupling
Drug dosages
Ferric chloride
Hydrazones
Hypertension
Iron chlorides
Laboratories
Linearity
Pharmaceuticals
Pulmonary hypertension
Regression analysis
Spectrophotometry
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Summary:The present study is a first report on development of a spectrophotometric method for determination of selexipag (used to cure pulmonary arterial hypertension) in bulk and tablet formulation and its validation. The basis of the proposed method is formation of a chromophore (of λ max 600 nm) in presence of acidic ferric chloride by oxidative coupling reaction between selexipag and MBTH (3-methylbenzo-thiazolin-2-one hydrazone) solution. Regression analysis (r > 0.999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (5 – 30 μg mL-1). As per the existing guidelines of ICH, various parameters of the method were tested for validation. Low values of R.S.D. (< 2 %) were observed indicating that the proposed method is reproducible, accurate and precise.
ISSN:0970-020X
2231-5039
DOI:10.13005/ojc/340656