Horseradish peroxidase-catalyzed hydrogelation consuming enzyme-produced hydrogen peroxide in the presence of reducing sugars

In the present work, three kinds of reducing sugars: glucose, galactose, and mannose, are applied to horseradish peroxidase (HRP)-catalyzed hydrogelation of an aqueous solution containing natural polymers modified with phenolic hydroxyl moieties. In this system, HRP consumes hydrogen peroxide that w...

Full description

Saved in:
Bibliographic Details
Published in:Soft matter 2019-03, Vol.15 (1), p.2163-2169
Main Authors: Gantumur, Enkhtuul, Sakai, Shinji, Nakahata, Masaki, Taya, Masahito
Format: Article
Language:English
Subjects:
Aqueous solutions
Biocompatibility
Galactose
Horseradish peroxidase
Hydrogels
Hydrogen peroxide
Mannose
Mechanical properties
Natural polymers
Oxidation
Peroxidase
Phenolic compounds
Phenols
Polymers
Reaction mechanisms
Stability
Sugar
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present work, three kinds of reducing sugars: glucose, galactose, and mannose, are applied to horseradish peroxidase (HRP)-catalyzed hydrogelation of an aqueous solution containing natural polymers modified with phenolic hydroxyl moieties. In this system, HRP consumes hydrogen peroxide that was generated from the oxidation of thiol groups in HRP in the presence of reducing sugars. Herein, we highlight the versatility of applicable sugar types and the controllable hydrogel properties. The mechanical properties and microstructures of the resultant hydrogels can be well controlled by varying the concentration and the reducing power of sugars. Moreover, reducing sugar-independent cytocompatibility of the hydrogels was confirmed by the growth of cells on them. The wide selection of sugar types provides a better understanding of the reaction mechanism and enables the characterization of hydrogels with well-controlled properties. Three kinds of reducing sugars are applied to HRP-catalyzed hydrogelation to promote the generation of H 2 O 2 in the reaction system.
ISSN:1744-683X
1744-6848
DOI:10.1039/c8sm01839a