Spent pot liner from aluminum industry: genotoxic and mutagenic action on human leukocytes

• Published in: Environmental science and pollution research international 2019-09, Vol.26 (27), p.27640-27646
• Main Authors: Andrade-Vieira, Larissa Fonseca, Trento, Marcus Vinícius Cardoso, César, Pedro Henrique Souza, Marcussi, Silvana
• Format: Article
• Language: English
• Subjects: Adult; Aluminum; Aluminum - toxicity; Analytical Methods; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bioassays; Calcium chloride; Cell Nucleus - drug effects; Comet Assay; Damage detection; Deoxyribonucleic acid; Dependence; DNA; DNA Damage; Doxorubicin; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental Samples; Environmental science; Exposure; Genotoxic chemicals; Genotoxicity; Global Toxicity Assessment: Chemicals; Hazardous wastes; Humans; Leukocytes; Leukocytes - drug effects; Micronucleus Tests; Mining; Mutagenesis; Mutagens - toxicity; Nucleoids; Processing industry; Solid wastes; Toxic wastes; Toxicology; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-019-04782-2

Spent pot liner (SPL) is a toxic solid waste generated in the aluminum mining and processing industry. SPL is considered as an environmental pollution agent when is dumped on environment. Thus, it is important to access its toxicological risk for the exposed organisms. The comet assay and micronucleus test are efficient tests to detect genotoxic/mutagenic compounds by DNA damage observation. Therefore, in the present study, the genotoxic potential of SPL was evaluated through the micronucleus and comet assay on human leukocytes. After ethics committee approval (COEP—UFLA n°. CAAE 11355312.8.0000.5060), blood aliquots collected from healthy volunteers were exposed to increasing concentrations of SPL (from 0.1 to 80 g L −1 ). All SPL treatments, including the lowest concentration applied (0.1 g L −1 ), significantly increased the micronucleus frequency. The frequency of DNA damage was determined by visual scores (from 0 to 4) and the results were expressed on percentage of damage and arbitrary units (AU). CaCl 2 (0.01 M) was applied as negative control (NC) and doxorubicin (10 μg mL −1 ) as positive control (PC). It was observed a dose-dependency between SPL treatments: as SPL concentration for cell incubation increases, the frequency of damage on DNA also increases. Cells incubated on the NC presented nucleoids class 0 to 2, while those exposed to SPL presents nucleoids class 0 to 4. SPL-incubated cells increasing significantly the frequency of nucleoids class 4. For the PC, the UA of damage was 267.74, which is lower than the one observed for the treatments with high doses of SPL (40–287.40 g L −1 and 80–315.30 g L −1 ). Thus, it was demonstrated that the SPL is a genotoxic agent that induces DNA damage on exposed organisms.