A ratiometric fluorescent probe for lysosomal hypochlorous acid based on through-bond energy transfer strategy

In this paper, we synthesized a ratiometric fluorescence probe (IRh-Ly) for lysosomal hypochlorous acid (HOCl) by adopting a through-bond energy transfer (TBET) strategy on rhodamine-imidazo[1,5-a]pyridine platform. IRh-Ly showed brilliant selectivity, rapid response for HOCl. The probe also exhibit...

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Bibliographic Details
Published in:Analytica chimica acta 2019-04, Vol.1052, p.124-130
Main Authors: Shen, Shi-Li, Huang, Xiao-Qing, Lin, Xiao-Hui, Cao, Xiao-Qun
Format: Article
Language:English
Subjects:
Animals
Bond energy
Energy Transfer
Fluorescence
Fluorescent Dyes - metabolism
Fluorescent indicators
Fluorescent probe
HOCl
Hypochlorous acid
Hypochlorous Acid - metabolism
Lysosomal
Lysosomes - metabolism
Mice
Morpholine
Morpholines - chemistry
Morpholines - metabolism
Optical Imaging
Pyridines
Ratiometric
RAW 264.7 Cells
Rhodamine
Selectivity
Target detection
Through-bond energy transfer
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Summary:In this paper, we synthesized a ratiometric fluorescence probe (IRh-Ly) for lysosomal hypochlorous acid (HOCl) by adopting a through-bond energy transfer (TBET) strategy on rhodamine-imidazo[1,5-a]pyridine platform. IRh-Ly showed brilliant selectivity, rapid response for HOCl. The probe also exhibited high sensitivity with the detection limit calculated to be 10.2 nM. Moreover, we demonstrated its successful application of detecting lysosomal HOCl in living RAW264.7 cells. Notably, the morpholine was integrated into the fluorescent probe IRh-Ly and the results revealed that IRh-Ly could target lysosome and detect the lysosomal HOCl. All the unique features made IRh-Ly particularly suitable for ratiometric HOCl detection and bio-imaging applications. [Display omitted] •A novel ratiometric HOCl fluorescent probe was developed based on the imidazo[1,5-a]pyridine -rhodamine TBET platform.•The probe showed superior selectivity and sensitivity toward HOCl.•The probe could selectively stain lysosome.•The probe was suitable for imaging endogenous HOCl in RAW264.7 cells.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2018.11.030