Efficient docosahexaenoic acid production by Schizochytrium sp. via a two-phase pH control strategy using ammonia and citric acid as pH regulators

[Display omitted] •An efficient docosahexaenoic acid (DHA) production strategy was developed.•A two-phase pH control strategy promoted cell growth and DHA synthesis.•Ammonia was used as both nitrogen source and pH regulator.•Citric acid as an acidic pH regulator increased the DHA percentage in total...

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Bibliographic Details
Published in:Process biochemistry (1991) 2019-02, Vol.77, p.1-7
Main Authors: Yin, Feng-Wei, Zhang, Yu-Ting, Jiang, Jia-Yi, Guo, Dong-Sheng, Gao, Song, Gao, Zhen
Format: Article
Language:English
Subjects:
Accumulation
Acid production
Ammonia
Biomass
Cell culture
Cell density
Citric acid
Docosahexaenoic acid
Fatty acids
Fermentation
Fish oils
Industrial production
Lipids
Nitrogen
pH control
pH effects
Regulators
Schizochytrium
Schizochytrium sp
Strategy
Synthesis
Two-phase pH
Weight
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Summary:[Display omitted] •An efficient docosahexaenoic acid (DHA) production strategy was developed.•A two-phase pH control strategy promoted cell growth and DHA synthesis.•Ammonia was used as both nitrogen source and pH regulator.•Citric acid as an acidic pH regulator increased the DHA percentage in total fatty acids.•This work offers new perspectives on high-cell-density fermentation. The cell growth and docosahexaenoic acid (DHA) synthesis of Schizochytrium sp. are closely related to the culture pH. A two-phase pH control strategy based on nitrogen consumption was developed in which pH 7.0 was used for biomass accumulation and pH 5.0 for DHA synthesis. Using this strategy, the cell dry weight and DHA content reached 98.07 and 25.85 g/L, respectively. Furthermore, ammonia and citric acid were used as pH regulators. Application of citric acid further resulted in 7.88 and 4.87% improvements of total lipids and the ratio of DHA to total fatty acids, respectively. Ammonia, as a suitable nitrogen source, promoted non-lipid biomass accumulation. Using this method, a maximum DHA yield of 32.75 g/L was obtained with non-lipid biomass (58.01 g/L) and the ratio of DHA to total fatty acids (52.36%). This study provides an easy strategy for large-scale industrial production of DHA via high-cell-density fermentation of Schizochytrium sp.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2018.11.013