Detection of Antioxidant and Antimicrobial Activity of Leaf Extract of Jasminum azoricum

[...]this leaf extract can be used for the production of novel bioactive substance which can induce antioxidant and antimicrobial defence in humans and can thereby serve to eliminate human ailments. The radical scavenging activity of the sample was calculated by the following formula13: (ProQuest: ....

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2018-08, Vol.11 (8), p.3629-3632
Main Authors: Thiruvengadam, S., Nivedha, S., Pujita, V., Romauld, S. Ivo
Format: Article
Language:English
Subjects:
Antimicrobial agents
Antioxidants
Flavonoids
Free radicals
Herbal medicine
Leaves
Phenols
Phytochemicals
Steroids
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Summary:[...]this leaf extract can be used for the production of novel bioactive substance which can induce antioxidant and antimicrobial defence in humans and can thereby serve to eliminate human ailments. The radical scavenging activity of the sample was calculated by the following formula13: (ProQuest: ... denotes formula omitted.) QUANTITATIVE ASSAY OF ANTI-OXIDANT ACTIVITY: 100pl of acetone leaf extract sample from qualitative assay were mixed with 2.7ml of Methanol followed by the addition of 200pl of 0.1% methanolic DPPH. Different concentrations (10mg, 20mg, and 30mg/ml) of the concentrated acetone leaf extract were tested for their antimicrobial activity against different pathogenic bacterial strains such as Staphylococcus aureus17, Bacillus cereus, Bacillus subtilis, Escherichia coli and Pseudomonas sp. The column chromatography eluted 9 different fractions and the F5 fraction showed higher anti-oxidant activity of 66.07% when compared to other fractions.The minimum antioxidant activity was found to be 56.25% for F2 fraction. [...]the F5 fraction was preferred for antimicrobial activity.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.5958/0974-360X.2018.00668.6