Colorimetric Analysis of Raltegravir in Pharmaceutical Formulation and Human Biological Fluids through Oxidative Coupling Reaction using 3-Methyl-2-Benzothiazolinone Hydrazone Reagent

After thorough survey, there is no colorimetric method was reported about this drug using MBTH reagent. [...]an effort was created for the estimation of Raltegravir by colorimetric method using MBTH reagent. The drug-excipient interference studies were investigated by examining the interaction betwe...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2018-05, Vol.11 (5), p.1788-1793
Main Authors: Panigrahy, Uttam Prasad, Panda, Siva Prasad, Sudhakar, Muvvala
Format: Article
Language:English
Subjects:
Antiretroviral drugs
Chloride
Chromatography
Enzymes
HIV
Human immunodeficiency virus
Mass spectrometry
Pharmaceutical sciences
Potassium
Scientific imaging
Solvents
Urine
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Summary:After thorough survey, there is no colorimetric method was reported about this drug using MBTH reagent. [...]an effort was created for the estimation of Raltegravir by colorimetric method using MBTH reagent. The drug-excipient interference studies were investigated by examining the interaction between the drug and generally encountered excipients such as microcrystalline cellulose, lactose, eudragit, magnesium stearate and coating agent like opadry II pink. 100 pg/mL of Raltegravir pure drug was added separately with increasing concentration of potential excipients followed by the addition of MBTH-ferric chloride-HCl solution and kept aside for one hour for complete reaction to produce colored complex. Tsuchiya K, Ohuchi M, Yamane N, Aikawa H, Gatanaga H, Oka S, Hamada A. High-performance liquid chromatography-tandem mass spectrometry for simultaneous determination of raltegravir, dolutegravir and elvitegravir concentrations in human plasma and cerebrospinal fluid samples. Quaranta S, Woloch C, Paccou A, Giocanti M, Solas C, Lacarelle B. Validation of an electrospray ionization LC-MS/MS method for quantitative analysis of raltegravir, etravirine, and 9 other antiretroviral agents in human plasma samples.
ISSN:0974-3618
0974-306X
DOI:10.5958/0974-360X.2018.00332.3