Changes in FAT/CD36, UCP2, UCP3 and GLUT4 gene expression during lipid infusion in rat skeletal and heart muscle

Objective: It has been reported that an increased availability of free fatty acids (NEFA) not only interferes with glucose utilization in insulin-dependent tissues, but may also result in an uncoupling effect of heart metabolism. We aimed therefore to investigate the effect of an increased availabil...

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Bibliographic Details
Published in:International Journal of Obesity 2002-06, Vol.26 (6), p.838-847
Main Authors: Vettor, R, Fabris, R, Serra, R, Lombardi, A.M, Tonello, C, Granzotto, M, Marzolo, M.O, Carruba, M.O, Ricquier, D, Federspil, G, Nisoli, E
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Biological and medical sciences
Catheters
Diabetes
experimental design
Fatty acids
Feeding. Feeding behavior
free fatty acids
Fundamental and applied biological sciences. Psychology
Gene expression
Glucose
glucose clamp technique
Heart
heparin
Insulin
Intermediate and energetic metabolism
Laboratory animals
Lipids
messenger RNA
Metabolism
Metabolisms and neurohumoral controls
muscles
Musculoskeletal system
Northern blotting
Obesity
Oxidation
Proteins
rats
reverse transcriptase polymerase chain reaction
skeletal muscle
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Objective: It has been reported that an increased availability of free fatty acids (NEFA) not only interferes with glucose utilization in insulin-dependent tissues, but may also result in an uncoupling effect of heart metabolism. We aimed therefore to investigate the effect of an increased availability of NEFA on gene expression of proteins involved in transmembrane fatty acid (FAT/CD36) and glucose (GLUT4) transport and of the uncoupling proteins UCP2 and 3 at the heart and skeletal muscle level. Study Design: Euglycemic hyperinsulinemic clamp was performed after 24 h Intralipid plus heparin or saline infusion in lean Zucker rats. Skeletal and heart muscle glucose utilization was calculated by 2-deoxy-[1-3H]-D-glucose technique. Quantification of FAT/CD36, GLUT4, UCP2 and UCP3 mRNAs was obtained by Northern blot analysis or RT-PCR. Results: In Intralipid plus heparin infused animals a significant decrease in insulin-mediated glucose uptake was observed both in the heart (22.62±2.04 vs 10.37±2.33 ng/mg/min; P
ISSN:0307-0565
1476-5497
DOI:10.1038/sj.ijo.0802005