Designing and Development of Rice water based crude media and it's application in fungal isolation and enzyme production

• Published in: Research journal of pharmacy and technology 2018-09, Vol.11 (9), p.3905-3908
• Main Authors: Thiruvengadam, S., Jeevanantham, S., Kamalesh, R., Hamsini, S, Kamali, T. B, Karishma, S., Jayalakshmi, H.
• Format: Article
• Language: English
• Subjects: Antibiotics; Carbohydrates; Dietary minerals; Enzymes; Fermentation; Food science; Fungi; Iodine; Morphology; Nutrition; Rice
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.5958/0974-360X.2018.00716.3

The genus is found worldwide and comprises around 180 officially recognized species and are useful microorganisms in nature for degradation of plant polysaccharides.3,4 They have important industrial application in the large-scale production of both homologous and heterologous enzymes.5 6 Among them, Aspergillus oryzae and Aspergillus niger are on the Generally Recognized as Safe (GRAS) listed by Food and Drug Administration (FDA) in the United State7. The production of amylase by fungi has been greatly affected by cultural and nutritional requirements. [...]the results of the present study will provide awareness in fungal isolation and production of amylase by aiding the development of a feasible medium and characterization of amylase. [...]the identified fungal strains were sub cultured in fresh rice water agar medium for further growth and characterisation. Function of amylase activity is measured by the formation of reducing sugar due to enzymatic hydrolysis of starch by the method described by Bernfeld(1955).13 1 ml of crude enzyme extract and citrate phosphate buffer (pH 6.5) containing 1 ml of 1% (w/v) starch forms a reaction mixture which was incubated at 40° C for half an hour and the reaction was arrested by addition of 2ml of DNS.