Phanerochaete chrysosporium as a model organism to assess the toxicity of municipal landfill leachate from Elazığ, Turkey

• Published in: Environmental science and pollution research international 2019-05, Vol.26 (13), p.12807-12812
• Main Authors: Yildirim, Numan, Yildirim, Nuran Cikcikoglu, Tatar, Sule, Alp, Hevidar
• Format: Article
• Language: English
• Subjects: Antioxidants; Antioxidants - metabolism; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biological Oxygen Demand Analysis; Catalase; Catalase - metabolism; Chemical oxygen demand; Dextrose; Dilution; Dissolved oxygen; Earth and Environmental Science; Ecological effects; Ecotoxicology; Ecotoxicology - methods; Environment; Environmental Chemistry; Environmental Health; Environmental science; Enzyme-linked immunosorbent assay; Glutathione; Glutathione - metabolism; Hydrogen-Ion Concentration; Landfill; Landfills; Leachates; Municipal landfills; Municipal solid waste; Organic chemistry; Oxidation-Reduction; Oxidative stress; Oxidative Stress - drug effects; Parameters; Phanerochaete - drug effects; Phanerochaete - metabolism; Phanerochaete chrysosporium; Physiochemistry; Research Article; Superoxide dismutase; Superoxide Dismutase - metabolism; Total dissolved solids; Toxicity; Turkey; Waste disposal sites; Waste Water Technology; Water Management; Water Pollutants, Chemical - chemistry; Water Pollutants, Chemical - toxicity; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-019-04813-y

In order to evaluate the potential ecological risk and the toxic effect of landfill leachate (LL), Phanerochaete chrysosporium was exposed to LL and their biochemical response was observed by using antioxidant parameters. Phanerochaete chrysosporium , ME 446, was kept at 4 °C after being sub-cultured at 28 °C on Sabouraud Dextrose Agar (SDA). Superoxide dismutase (SOD), catalase (CAT) activities, and malaondialdehyde (MDA) and glutathione (GSH) levels of P. chrysosporium exposed to different dilution rates of leachate (1/10 and 1/20) for 24 and 96 h were analyzed by using the ELISA method. The physiochemical parameters such as pH, conductivity, total dissolved solids (TDS), dissolved oxygen (DO), chemical oxygen demand (COD) of leachate, and reference water were analyzed by using the YSI Professional Plus handheld multiparameter meter. In this study, SOD activities were decreased in the application groups compared with the Control Group at the 24th and 96th hours. CAT activities and GSH levels increased in the application groups compared with the Control Group at the 24th hour but decreased at the 96th hours. MDA levels increased in all of the application groups when compared with the Control Group for both 24 and 96 h. Different concentration of LL induces oxidative stress in P. chrysosporium , increased CAT activity and MDA levels, and decreased SOD activity and GSH levels.