Defining the Functional Role of Na V 1.7 in Human Nociception

• Published in: Neuron (Cambridge, Mass.) Mass.), 2019-03, Vol.101 (5), p.905
• Main Authors: McDermott, Lucy A, Weir, Greg A, Themistocleous, Andreas C, Segerdahl, Andrew R, Blesneac, Iulia, Baskozos, Georgios, Clark, Alex J, Millar, Val, Peck, Liam J, Ebner, Daniel, Tracey, Irene, Serra, Jordi, Bennett, David L
• Format: Article
• Language: English
• Subjects: Action Potentials; Adult; Analgesics; Animal models; Axons - metabolism; Biopsy; Brain research; Capsaicin; Cell Line; Cells, Cultured; Clinical trials; Congenital defects; Congenital diseases; Consortia; Cortex; Depolarization; Diabetes; Diabetic neuropathy; Drug development; Epitopes; Excitability; Female; Functional magnetic resonance imaging; Genomes; Genotype & phenotype; Humans; Induced Pluripotent Stem Cells - metabolism; Induced Pluripotent Stem Cells - physiology; Innovations; Male; Microneurography; Mutation; NAV1.7 Voltage-Gated Sodium Channel - genetics; NAV1.7 Voltage-Gated Sodium Channel - metabolism; Nervous system; Neurons; Neurosciences; Nociception; Nociceptors; Nociceptors - metabolism; Nociceptors - pathology; Nociceptors - physiology; Nodes of Ranvier; Pain; Pain Insensitivity, Congenital - genetics; Pain Insensitivity, Congenital - metabolism; Pain Insensitivity, Congenital - physiopathology; Pain perception; Pluripotency; Presynapse; Ranvier's Nodes - metabolism; Skin; Sodium Channel Blockers - pharmacology; Sodium channels (voltage-gated); Stem cells
• ISSN: 0896-6273; 1097-4199
• DOI: 10.1016/j.neuron.2019.01.047

Loss-of-function mutations in Na 1.7 cause congenital insensitivity to pain (CIP); this voltage-gated sodium channel is therefore a key target for analgesic drug development. Utilizing a multi-modal approach, we investigated how Na 1.7 mutations lead to human pain insensitivity. Skin biopsy and microneurography revealed an absence of C-fiber nociceptors in CIP patients, reflected in a reduced cortical response to capsaicin on fMRI. Epitope tagging of endogenous Na 1.7 revealed the channel to be localized at the soma membrane, axon, axon terminals, and the nodes of Ranvier of induced pluripotent stem cell (iPSC) nociceptors. CIP patient-derived iPSC nociceptors exhibited an inability to properly respond to depolarizing stimuli, demonstrating that Na 1.7 is a key regulator of excitability. Using this iPSC nociceptor platform, we found that some Na 1.7 blockers undergoing clinical trials lack specificity. CIP, therefore, arises due to a profound loss of functional nociceptors, which is more pronounced than that reported in rodent models, or likely achievable following acute pharmacological blockade. VIDEO ABSTRACT.