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Ochrobactrum cytisi IPA7.2 promotes growth of potato microplants and is resistant to abiotic stress
Bacteria in natural associations with agricultural crops are promising for use in the improvement of clonal micropropagation of plants. We clarified the taxonomic position of Ochrobactrum cytisi strain IPA7.2 and investigated its tolerance for salinity, high temperature, and glyphosate pollution. We...
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| Published in: | World journal of microbiology & biotechnology 2019-04, Vol.35 (4), p.55-12, Article 55 |
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| creator | Burygin, Gennady L. Kargapolova, Kristina Yu Kryuchkova, Yelena V. Avdeeva, Elena S. Gogoleva, Natalia E. Ponomaryova, Tatyana S. Tkachenko, Oksana V. |
| description | Bacteria in natural associations with agricultural crops are promising for use in the improvement of clonal micropropagation of plants. We clarified the taxonomic position of
Ochrobactrum cytisi
strain IPA7.2 and investigated its tolerance for salinity, high temperature, and glyphosate pollution. We also tested the strain’s potential to promote the growth of potato (
Solanum tuberosum
L.) microplants. Using the IPA7.2 draft genome (no. NZ_MOEC00000000), we searched for housekeeping genes and also for the target genes encoding glyphosate tolerance and plant-growth-promoting ability. A multilocus sequence analysis of the
gap, rpoB, dnaK, trpE, aroC
, and
recA
housekeeping genes led us to identify isolate IPA7.2 as
O. cytisi
. The strain tolerated temperatures up to 50 °C and NaCl concentrations up to 3–4%, and it produced 8 µg ml
−1
of indole-3-acetic acid. It also tolerated 6 mM glyphosate owing to the presence of type II 5-enolpyruvylshikimate-3-phosphate synthase. Finally, it was able to colonize the roots and tissues of potato microplants, an ability preserved by several generations after subculturing. We identified the development phase of potato microplants that was optimal for inoculation with
O. cytisi
IPA7.2. Inoculation of in vitro-grown 15-day-old microplants increased the mitotic index of root meristem cells (by 50%), the length of shoots (by 34%), the number of leaves (by 7%), and the number of roots (by 16%). Under ex vitro conditions, the inoculated plants had a greater leaf area (by 77%) and greater shoot and root dry weight (by 84 and 61%, respectively) than did the control plants. We recommend
O. cytisi
IPA 7.2 for use in the growing of potato microplants to improve the production of elite seed material.
Graphical abstract |
| doi_str_mv | 10.1007/s11274-019-2633-x |
| format | article |
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Ochrobactrum cytisi
strain IPA7.2 and investigated its tolerance for salinity, high temperature, and glyphosate pollution. We also tested the strain’s potential to promote the growth of potato (
Solanum tuberosum
L.) microplants. Using the IPA7.2 draft genome (no. NZ_MOEC00000000), we searched for housekeeping genes and also for the target genes encoding glyphosate tolerance and plant-growth-promoting ability. A multilocus sequence analysis of the
gap, rpoB, dnaK, trpE, aroC
, and
recA
housekeeping genes led us to identify isolate IPA7.2 as
O. cytisi
. The strain tolerated temperatures up to 50 °C and NaCl concentrations up to 3–4%, and it produced 8 µg ml
−1
of indole-3-acetic acid. It also tolerated 6 mM glyphosate owing to the presence of type II 5-enolpyruvylshikimate-3-phosphate synthase. Finally, it was able to colonize the roots and tissues of potato microplants, an ability preserved by several generations after subculturing. We identified the development phase of potato microplants that was optimal for inoculation with
O. cytisi
IPA7.2. Inoculation of in vitro-grown 15-day-old microplants increased the mitotic index of root meristem cells (by 50%), the length of shoots (by 34%), the number of leaves (by 7%), and the number of roots (by 16%). Under ex vitro conditions, the inoculated plants had a greater leaf area (by 77%) and greater shoot and root dry weight (by 84 and 61%, respectively) than did the control plants. We recommend
O. cytisi
IPA 7.2 for use in the growing of potato microplants to improve the production of elite seed material.
Graphical abstract</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-019-2633-x</identifier><identifier>PMID: 30900049</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject><![CDATA[Acetic acid ; Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; DnaK protein ; Environmental Engineering/Biotechnology ; Genes ; Genes, Bacterial - genetics ; Genes, Essential - genetics ; Genomes ; Glycine - adverse effects ; Glycine - analogs & derivatives ; Glyphosate ; High temperature ; Indoleacetic acid ; Indoleacetic Acids - metabolism ; Inoculation ; Leaf area ; Leaves ; Life Sciences ; Microbiology ; Micropropagation ; Multilocus Sequence Typing ; Ochrobactrum ; Ochrobactrum - classification ; Ochrobactrum - genetics ; Ochrobactrum - isolation & purification ; Ochrobactrum - physiology ; Original Paper ; Phylogeny ; Plant Development ; Plant Leaves - growth & development ; Plant Leaves - microbiology ; Plant Roots - growth & development ; Plant Roots - microbiology ; Plant Shoots - growth & development ; Plant Shoots - microbiology ; Potatoes ; RecA protein ; RNA, Ribosomal, 16S - genetics ; Roots ; RpoB protein ; Salinity ; Salt Tolerance ; Shoots ; Sodium Chloride ; Soil Microbiology ; Solanum tuberosum - growth & development ; Solanum tuberosum - microbiology ; Stress, Physiological ; Temperature tolerance ; Thermotolerance ; Vegetables ; Weight]]></subject><ispartof>World journal of microbiology & biotechnology, 2019-04, Vol.35 (4), p.55-12, Article 55</ispartof><rights>Springer Nature B.V. 2019</rights><rights>World Journal of Microbiology and Biotechnology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-45fd4849051c09a8660bfd7ee90699a5559493c9808d787628802fe4630437f43</citedby><cites>FETCH-LOGICAL-c409t-45fd4849051c09a8660bfd7ee90699a5559493c9808d787628802fe4630437f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2195087262/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2195087262?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,11688,27924,27925,36060,44363,74895</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30900049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burygin, Gennady L.</creatorcontrib><creatorcontrib>Kargapolova, Kristina Yu</creatorcontrib><creatorcontrib>Kryuchkova, Yelena V.</creatorcontrib><creatorcontrib>Avdeeva, Elena S.</creatorcontrib><creatorcontrib>Gogoleva, Natalia E.</creatorcontrib><creatorcontrib>Ponomaryova, Tatyana S.</creatorcontrib><creatorcontrib>Tkachenko, Oksana V.</creatorcontrib><title>Ochrobactrum cytisi IPA7.2 promotes growth of potato microplants and is resistant to abiotic stress</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>World J Microbiol Biotechnol</addtitle><description>Bacteria in natural associations with agricultural crops are promising for use in the improvement of clonal micropropagation of plants. We clarified the taxonomic position of
Ochrobactrum cytisi
strain IPA7.2 and investigated its tolerance for salinity, high temperature, and glyphosate pollution. We also tested the strain’s potential to promote the growth of potato (
Solanum tuberosum
L.) microplants. Using the IPA7.2 draft genome (no. NZ_MOEC00000000), we searched for housekeeping genes and also for the target genes encoding glyphosate tolerance and plant-growth-promoting ability. A multilocus sequence analysis of the
gap, rpoB, dnaK, trpE, aroC
, and
recA
housekeeping genes led us to identify isolate IPA7.2 as
O. cytisi
. The strain tolerated temperatures up to 50 °C and NaCl concentrations up to 3–4%, and it produced 8 µg ml
−1
of indole-3-acetic acid. It also tolerated 6 mM glyphosate owing to the presence of type II 5-enolpyruvylshikimate-3-phosphate synthase. Finally, it was able to colonize the roots and tissues of potato microplants, an ability preserved by several generations after subculturing. We identified the development phase of potato microplants that was optimal for inoculation with
O. cytisi
IPA7.2. Inoculation of in vitro-grown 15-day-old microplants increased the mitotic index of root meristem cells (by 50%), the length of shoots (by 34%), the number of leaves (by 7%), and the number of roots (by 16%). Under ex vitro conditions, the inoculated plants had a greater leaf area (by 77%) and greater shoot and root dry weight (by 84 and 61%, respectively) than did the control plants. We recommend
O. cytisi
IPA 7.2 for use in the growing of potato microplants to improve the production of elite seed material.
Graphical abstract</description><subject>Acetic acid</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>DnaK protein</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Genes</subject><subject>Genes, Bacterial - genetics</subject><subject>Genes, Essential - genetics</subject><subject>Genomes</subject><subject>Glycine - adverse effects</subject><subject>Glycine - analogs & derivatives</subject><subject>Glyphosate</subject><subject>High temperature</subject><subject>Indoleacetic acid</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Inoculation</subject><subject>Leaf area</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Micropropagation</subject><subject>Multilocus Sequence Typing</subject><subject>Ochrobactrum</subject><subject>Ochrobactrum - classification</subject><subject>Ochrobactrum - genetics</subject><subject>Ochrobactrum - isolation & purification</subject><subject>Ochrobactrum - physiology</subject><subject>Original Paper</subject><subject>Phylogeny</subject><subject>Plant Development</subject><subject>Plant Leaves - growth & development</subject><subject>Plant Leaves - microbiology</subject><subject>Plant Roots - growth & development</subject><subject>Plant Roots - microbiology</subject><subject>Plant Shoots - growth & development</subject><subject>Plant Shoots - microbiology</subject><subject>Potatoes</subject><subject>RecA protein</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Roots</subject><subject>RpoB protein</subject><subject>Salinity</subject><subject>Salt Tolerance</subject><subject>Shoots</subject><subject>Sodium Chloride</subject><subject>Soil Microbiology</subject><subject>Solanum tuberosum - growth & development</subject><subject>Solanum tuberosum - microbiology</subject><subject>Stress, Physiological</subject><subject>Temperature tolerance</subject><subject>Thermotolerance</subject><subject>Vegetables</subject><subject>Weight</subject><issn>0959-3993</issn><issn>1573-0972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNp1kE1LAzEQhoMotlZ_gBcJeF6dfOxm51iKXyDUg55Dms22K92mJinaf29Kq548Dcy87zszDyGXDG4YgLqNjHElC2BY8EqI4uuIDFmpRAGo-DEZApZYCEQxIGcxvgNkF4pTMhCAACBxSOzULoKfGZvCpqd2m7rY0aeXsbrhdB1875OLdB78Z1pQ39K1TyZ52nc2-PXSrFKkZtXQLtLgYhdT7tA8N7POp87SmHI7npOT1iyjuzjUEXm7v3udPBbP04enyfi5sBIwFbJsG1lLhJJZQFNXFczaRjmHUCGasixRorBYQ92oWlW8roG3TlYCpFCtFCNyvc_Nh39sXEz63W_CKq_UnGEJteIVzyq2V-UXYgyu1evQ9SZsNQO9w6r3WHXGqndY9Vf2XB2SN7PeNb-OH45ZwPeCmEeruQt_q_9P_QaaNIJC</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Burygin, Gennady L.</creator><creator>Kargapolova, Kristina Yu</creator><creator>Kryuchkova, Yelena V.</creator><creator>Avdeeva, Elena S.</creator><creator>Gogoleva, Natalia E.</creator><creator>Ponomaryova, Tatyana S.</creator><creator>Tkachenko, Oksana V.</creator><general>Springer Netherlands</general><general>Springer Nature 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cytisi IPA7.2 promotes growth of potato microplants and is resistant to abiotic stress</title><author>Burygin, Gennady L. ; Kargapolova, Kristina Yu ; Kryuchkova, Yelena V. ; Avdeeva, Elena S. ; Gogoleva, Natalia E. ; Ponomaryova, Tatyana S. ; Tkachenko, Oksana V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-45fd4849051c09a8660bfd7ee90699a5559493c9808d787628802fe4630437f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetic acid</topic><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>DnaK protein</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Genes</topic><topic>Genes, Bacterial - genetics</topic><topic>Genes, Essential - genetics</topic><topic>Genomes</topic><topic>Glycine - adverse effects</topic><topic>Glycine - analogs & derivatives</topic><topic>Glyphosate</topic><topic>High temperature</topic><topic>Indoleacetic acid</topic><topic>Indoleacetic Acids - metabolism</topic><topic>Inoculation</topic><topic>Leaf area</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Micropropagation</topic><topic>Multilocus Sequence Typing</topic><topic>Ochrobactrum</topic><topic>Ochrobactrum - classification</topic><topic>Ochrobactrum - genetics</topic><topic>Ochrobactrum - isolation & purification</topic><topic>Ochrobactrum - physiology</topic><topic>Original Paper</topic><topic>Phylogeny</topic><topic>Plant Development</topic><topic>Plant Leaves - growth & development</topic><topic>Plant Leaves - microbiology</topic><topic>Plant Roots - growth & development</topic><topic>Plant Roots - microbiology</topic><topic>Plant Shoots - growth & development</topic><topic>Plant Shoots - microbiology</topic><topic>Potatoes</topic><topic>RecA protein</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Roots</topic><topic>RpoB protein</topic><topic>Salinity</topic><topic>Salt Tolerance</topic><topic>Shoots</topic><topic>Sodium Chloride</topic><topic>Soil Microbiology</topic><topic>Solanum tuberosum - growth & development</topic><topic>Solanum tuberosum - microbiology</topic><topic>Stress, Physiological</topic><topic>Temperature tolerance</topic><topic>Thermotolerance</topic><topic>Vegetables</topic><topic>Weight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burygin, Gennady L.</creatorcontrib><creatorcontrib>Kargapolova, Kristina Yu</creatorcontrib><creatorcontrib>Kryuchkova, Yelena V.</creatorcontrib><creatorcontrib>Avdeeva, Elena S.</creatorcontrib><creatorcontrib>Gogoleva, Natalia E.</creatorcontrib><creatorcontrib>Ponomaryova, Tatyana S.</creatorcontrib><creatorcontrib>Tkachenko, Oksana V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burygin, Gennady L.</au><au>Kargapolova, Kristina Yu</au><au>Kryuchkova, Yelena V.</au><au>Avdeeva, Elena S.</au><au>Gogoleva, Natalia E.</au><au>Ponomaryova, Tatyana S.</au><au>Tkachenko, Oksana V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ochrobactrum cytisi IPA7.2 promotes growth of potato microplants and is resistant to abiotic stress</atitle><jtitle>World journal of microbiology & biotechnology</jtitle><stitle>World J Microbiol Biotechnol</stitle><addtitle>World J Microbiol Biotechnol</addtitle><date>2019-04-01</date><risdate>2019</risdate><volume>35</volume><issue>4</issue><spage>55</spage><epage>12</epage><pages>55-12</pages><artnum>55</artnum><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>Bacteria in natural associations with agricultural crops are promising for use in the improvement of clonal micropropagation of plants. We clarified the taxonomic position of
Ochrobactrum cytisi
strain IPA7.2 and investigated its tolerance for salinity, high temperature, and glyphosate pollution. We also tested the strain’s potential to promote the growth of potato (
Solanum tuberosum
L.) microplants. Using the IPA7.2 draft genome (no. NZ_MOEC00000000), we searched for housekeeping genes and also for the target genes encoding glyphosate tolerance and plant-growth-promoting ability. A multilocus sequence analysis of the
gap, rpoB, dnaK, trpE, aroC
, and
recA
housekeeping genes led us to identify isolate IPA7.2 as
O. cytisi
. The strain tolerated temperatures up to 50 °C and NaCl concentrations up to 3–4%, and it produced 8 µg ml
−1
of indole-3-acetic acid. It also tolerated 6 mM glyphosate owing to the presence of type II 5-enolpyruvylshikimate-3-phosphate synthase. Finally, it was able to colonize the roots and tissues of potato microplants, an ability preserved by several generations after subculturing. We identified the development phase of potato microplants that was optimal for inoculation with
O. cytisi
IPA7.2. Inoculation of in vitro-grown 15-day-old microplants increased the mitotic index of root meristem cells (by 50%), the length of shoots (by 34%), the number of leaves (by 7%), and the number of roots (by 16%). Under ex vitro conditions, the inoculated plants had a greater leaf area (by 77%) and greater shoot and root dry weight (by 84 and 61%, respectively) than did the control plants. We recommend
O. cytisi
IPA 7.2 for use in the growing of potato microplants to improve the production of elite seed material.
Graphical abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30900049</pmid><doi>10.1007/s11274-019-2633-x</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2019-04, Vol.35 (4), p.55-12, Article 55 |
| issn | 0959-3993 1573-0972 |
| language | eng |
| recordid | cdi_proquest_journals_2195087262 |
| source | ABI/INFORM global; Springer Link |
| subjects | Acetic acid Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology DnaK protein Environmental Engineering/Biotechnology Genes Genes, Bacterial - genetics Genes, Essential - genetics Genomes Glycine - adverse effects Glycine - analogs & derivatives Glyphosate High temperature Indoleacetic acid Indoleacetic Acids - metabolism Inoculation Leaf area Leaves Life Sciences Microbiology Micropropagation Multilocus Sequence Typing Ochrobactrum Ochrobactrum - classification Ochrobactrum - genetics Ochrobactrum - isolation & purification Ochrobactrum - physiology Original Paper Phylogeny Plant Development Plant Leaves - growth & development Plant Leaves - microbiology Plant Roots - growth & development Plant Roots - microbiology Plant Shoots - growth & development Plant Shoots - microbiology Potatoes RecA protein RNA, Ribosomal, 16S - genetics Roots RpoB protein Salinity Salt Tolerance Shoots Sodium Chloride Soil Microbiology Solanum tuberosum - growth & development Solanum tuberosum - microbiology Stress, Physiological Temperature tolerance Thermotolerance Vegetables Weight |
| title | Ochrobactrum cytisi IPA7.2 promotes growth of potato microplants and is resistant to abiotic stress |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T09%3A31%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ochrobactrum%20cytisi%20IPA7.2%20promotes%20growth%20of%20potato%20microplants%20and%20is%20resistant%20to%20abiotic%20stress&rft.jtitle=World%20journal%20of%20microbiology%20&%20biotechnology&rft.au=Burygin,%20Gennady%20L.&rft.date=2019-04-01&rft.volume=35&rft.issue=4&rft.spage=55&rft.epage=12&rft.pages=55-12&rft.artnum=55&rft.issn=0959-3993&rft.eissn=1573-0972&rft_id=info:doi/10.1007/s11274-019-2633-x&rft_dat=%3Cproquest_cross%3E2195087262%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c409t-45fd4849051c09a8660bfd7ee90699a5559493c9808d787628802fe4630437f43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2195087262&rft_id=info:pmid/30900049&rfr_iscdi=true |