A simple immunosensor for alpha-fetoprotein determination based on gold nanoparticles-dextran-reduced graphene oxide

In this paper, a simple and sensitive immunosensor was fabricated for the detection of alpha-fetoprotein (AFP), which is based on a one-pot synthesized nanocomposite of gold nanoparticles-dextran-reduced graphene oxide (AuNPs-Dex-RGO). The AuNPs-Dex-RGO nanocomposite structure was confirmed by trans...

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Bibliographic Details
Published in:Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2019-01, Vol.833, p.126-132
Main Authors: Zhou, Jiexin, Zhang, Cong, Chen, Yuan, Wang, Zihua, Lan, Lintao, Wang, Yingying, Han, Bingkai, Pan, Meixin, Jiao, Jun, Chen, Qiang
Format: Article
Language:English
Subjects:
Alpha-fetoprotein
Dextran
Fourier transforms
Gold
Gold nanoparticles
Graphene
Immunosensor
Immunosensors
Infrared spectroscopy
Iron cyanides
Nanocomposites
Nanoparticles
Raman spectroscopy
Reproducibility
Selectivity
Spectrum analysis
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Summary:In this paper, a simple and sensitive immunosensor was fabricated for the detection of alpha-fetoprotein (AFP), which is based on a one-pot synthesized nanocomposite of gold nanoparticles-dextran-reduced graphene oxide (AuNPs-Dex-RGO). The AuNPs-Dex-RGO nanocomposite structure was confirmed by transmission electron microscope (TEM), Raman spectroscopy and Fourier transform infrared spectroscopy (FT-IR). With ferricyanide as the electrochemical probe, this method achieved ultrasensitive detection of AFP antigen. The specific immunoreaction between AFP and AFP antibody resulted in a linear relationship between the redox peak current intensity of [Fe(CN)6]4−/3- and AFP concentration. The detection range is 0.01–20ngmL−1, and the detection limit is 0.05pgmL−1. Moreover, the selectivity, reproducibility and stability of the proposed immunosensor were acceptable, which permitted the feasibility for the determination of AFP in human serum with satisfactory results, indicating the great potential for clinical application. •We proposed a new biosensor which novelly combines the advantages of both dextran and gold nanoparticles.•The AFP immunosensor has been successfully fabricated directly and simply by one-step synthesis based on AuNPs-Dex-RGO.•The AFP immunosensor exhibited outstanding performance with the wide linear range and low detection limit.•The AFP immunosensor has been successfully applied in the analysis of AFP in serum samples.•The AFP immunosensor exhibited high sensitivity and good stability.
ISSN:1572-6657
1873-2569
DOI:10.1016/j.jelechem.2018.11.036