Hae III-A Suitable Restriction Endonuclease for Restriction Fragment Length Polymorphism Analysis of Biological Evidence Samples

Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similar...

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Bibliographic Details
Published in:Journal of forensic sciences 1990-05, Vol.35 (3), p.530-536
Main Authors: Budowle, B, Waye, JS, Shutler, GG, Samuel Baechtel, F
Format: Article
Language:English
Subjects:
Base Sequence
Deoxyribonucleases, Type II Site-Specific
DNA - blood
Forensic hematology
Forensic Medicine - methods
Forensic sciences
Humans
Polymorphism, Restriction Fragment Length
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Summary:Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Finally, Hae III is a relatively inexpensive restriction endonuclease.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS12858J