Substitution of H2O for Formamide in the Sample Preparation Protocol for STR Analysis Using the Capillary Electrophoresis System: The Effects on Precision, Resolution, and Capillary Life

Water has been evaluated as a suitable solvent to replace formamide in the preparation of samples for capillary electrophoresis analysis. Critical aspects relevant to forensic analysis were studied to test the validity of the substitution. Correct assignment of allele labels was reproducibly perform...

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Bibliographic Details
Published in:Journal of forensic sciences 1999-09, Vol.44 (5), p.1029-1031
Main Authors: Biega, LA, Duceman, BW
Format: Article
Language:English
Subjects:
DNA fingerprints
Forensic sciences
Water
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Summary:Water has been evaluated as a suitable solvent to replace formamide in the preparation of samples for capillary electrophoresis analysis. Critical aspects relevant to forensic analysis were studied to test the validity of the substitution. Correct assignment of allele labels was reproducibly performed on known samples, and size determination was found to be precise. Three hundred injections, regardless the method of preparation, may be run on a single capillary without loss of precision or resolution.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS12034J