The Ability of Ixodid Ticks (Acari: Ixodidae) to Support Reproduction of the Tick-Borne Encephalitis Virus

Reproduction dynamics and phenotypic changes in the tick-borne encephalitis virus (TBEV) population during long-term infection, feeding of ixodid ticks, and at different temperatures were studied. The temperature significantly influenced the level of TBEV reproduction in ixodid ticks. However, more...

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Bibliographic Details
Published in:Entomological review 2018-12, Vol.98 (9), p.1369-1378
Main Authors: Belova, O. A., Kholodilov, I. S., Litov, A. G., Karganova, G. G.
Format: Article
Language:English
Subjects:
Arachnids
Biomedical and Life Sciences
Cell culture
Encephalitis
Feeding
Genetic vectors
Health aspects
Infection
Invasiveness
Life Sciences
Phenotypes
Population
Reproduction
Strains (organisms)
Tick-borne diseases
Tick-borne encephalitis
Vectors
Zoology
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Summary:Reproduction dynamics and phenotypic changes in the tick-borne encephalitis virus (TBEV) population during long-term infection, feeding of ixodid ticks, and at different temperatures were studied. The temperature significantly influenced the level of TBEV reproduction in ixodid ticks. However, more rapid and intensive TBEV reproduction was observed in feeding ticks as compared with infected ticks kept at elevated (32–37°C) and room temperatures. TBEV strains of the European and Siberian subtypes successfully replicated and formed long-term infection in ticks that are both primary ( Ixodes ricinus , I. persulcatus ) and secondary ( Dermacentor reticulatus ) virus vectors. The strain of the Siberian subtype reached its maximum titers in D. reticulatus , and the strain of the European subtype, in I. ricinus and D. reticulatus . Prolonged reproduction of the European and Siberian TBEV subtypes in both primary and secondary vectors increased heterogeneity of the virus population; however, virus variants retained high neuroinvasiveness characteristic of the virus adapted to mouse CNS cells. TBEV strains of different subtypes varied in the phenotypic population heterogeneity under different conditions. Unlike Absettarov (European subtype) strain, the population heterogeneity of EK-328 (Siberian subtype) strain by the plaque phenotype on PEK cell culture was significantly influenced by feeding of D. reticulatus ticks after long-term reproduction of the virus in them.
ISSN:0013-8738
1555-6689
DOI:10.1134/S0013873818090142