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Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk
Microsphere-based immunoassays involving flow cytometry have recently gained popularity for use in protein detection and infectious disease diagnosis due to their simple assay format and capacity for multiplexed analysis. In this work, four mycotoxins, including aflatoxin M 1 (AFM 1 ), ochratoxin A...
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| Published in: | Food analytical methods 2019-04, Vol.12 (4), p.877-886 |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
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| cited_by | cdi_FETCH-LOGICAL-c363t-47814c6aec24ade06697d74466669146ebac6cc357d018a2640b2cecf05e968c3 |
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| cites | cdi_FETCH-LOGICAL-c363t-47814c6aec24ade06697d74466669146ebac6cc357d018a2640b2cecf05e968c3 |
| container_end_page | 886 |
| container_issue | 4 |
| container_start_page | 877 |
| container_title | Food analytical methods |
| container_volume | 12 |
| creator | Qu, Jianwen Xie, Huijuan Zhang, Shuying Luo, Pengjie Guo, Ping Chen, Xianxiong Ke, Yuebin Zhuang, Junyu Zhou, Fangmei Jiang, Wenxiao |
| description | Microsphere-based immunoassays involving flow cytometry have recently gained popularity for use in protein detection and infectious disease diagnosis due to their simple assay format and capacity for multiplexed analysis. In this work, four mycotoxins, including aflatoxin M
1
(AFM
1
), ochratoxin A (OTA), deoxynivalenol (DON), and fumonisin B
1
(FB
1
), were selected as model analytes. A quadplex flow cytometric immunoassay (FCIA) was developed for detecting multiple mycotoxin residues in milk. The optimized quadplex FCIA exhibited satisfactory compatibility with the multiple mycotoxin residue analysis, with limits of detection (LODs) of 0.045 μg L
−1
for AFM
1
, 0.94 μg L
−1
for OTA, 7.48 μg L
−1
for DON, and 2.45 μg L
−1
for FB
1
. The recoveries of the target mycotoxins from spiked milk were 76–95%, with a relative standard deviation of less than 13.4%. Compared to traditional ELISA formats, the FCIA provided lower detection limits for multiple mycotoxin detection. |
| doi_str_mv | 10.1007/s12161-018-01412-4 |
| format | article |
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1
(AFM
1
), ochratoxin A (OTA), deoxynivalenol (DON), and fumonisin B
1
(FB
1
), were selected as model analytes. A quadplex flow cytometric immunoassay (FCIA) was developed for detecting multiple mycotoxin residues in milk. The optimized quadplex FCIA exhibited satisfactory compatibility with the multiple mycotoxin residue analysis, with limits of detection (LODs) of 0.045 μg L
−1
for AFM
1
, 0.94 μg L
−1
for OTA, 7.48 μg L
−1
for DON, and 2.45 μg L
−1
for FB
1
. The recoveries of the target mycotoxins from spiked milk were 76–95%, with a relative standard deviation of less than 13.4%. Compared to traditional ELISA formats, the FCIA provided lower detection limits for multiple mycotoxin detection.</description><identifier>ISSN: 1936-9751</identifier><identifier>EISSN: 1936-976X</identifier><identifier>DOI: 10.1007/s12161-018-01412-4</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aflatoxin M1 ; Aflatoxins ; Analytical Chemistry ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Deoxynivalenol ; Detection limits ; Enzyme-linked immunosorbent assay ; Flow cytometry ; Food Science ; Fumonisin B1 ; High-throughput screening ; Immunoassay ; Immunoassays ; Infectious diseases ; Microbiology ; Milk ; Multiplexing ; Mycotoxins ; Ochratoxin A ; Proteins ; Residues</subject><ispartof>Food analytical methods, 2019-04, Vol.12 (4), p.877-886</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-47814c6aec24ade06697d74466669146ebac6cc357d018a2640b2cecf05e968c3</citedby><cites>FETCH-LOGICAL-c363t-47814c6aec24ade06697d74466669146ebac6cc357d018a2640b2cecf05e968c3</cites><orcidid>0000-0003-2443-7648</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Qu, Jianwen</creatorcontrib><creatorcontrib>Xie, Huijuan</creatorcontrib><creatorcontrib>Zhang, Shuying</creatorcontrib><creatorcontrib>Luo, Pengjie</creatorcontrib><creatorcontrib>Guo, Ping</creatorcontrib><creatorcontrib>Chen, Xianxiong</creatorcontrib><creatorcontrib>Ke, Yuebin</creatorcontrib><creatorcontrib>Zhuang, Junyu</creatorcontrib><creatorcontrib>Zhou, Fangmei</creatorcontrib><creatorcontrib>Jiang, Wenxiao</creatorcontrib><title>Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk</title><title>Food analytical methods</title><addtitle>Food Anal. Methods</addtitle><description>Microsphere-based immunoassays involving flow cytometry have recently gained popularity for use in protein detection and infectious disease diagnosis due to their simple assay format and capacity for multiplexed analysis. In this work, four mycotoxins, including aflatoxin M
1
(AFM
1
), ochratoxin A (OTA), deoxynivalenol (DON), and fumonisin B
1
(FB
1
), were selected as model analytes. A quadplex flow cytometric immunoassay (FCIA) was developed for detecting multiple mycotoxin residues in milk. The optimized quadplex FCIA exhibited satisfactory compatibility with the multiple mycotoxin residue analysis, with limits of detection (LODs) of 0.045 μg L
−1
for AFM
1
, 0.94 μg L
−1
for OTA, 7.48 μg L
−1
for DON, and 2.45 μg L
−1
for FB
1
. The recoveries of the target mycotoxins from spiked milk were 76–95%, with a relative standard deviation of less than 13.4%. Compared to traditional ELISA formats, the FCIA provided lower detection limits for multiple mycotoxin detection.</description><subject>Aflatoxin M1</subject><subject>Aflatoxins</subject><subject>Analytical Chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Deoxynivalenol</subject><subject>Detection limits</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Flow cytometry</subject><subject>Food Science</subject><subject>Fumonisin B1</subject><subject>High-throughput screening</subject><subject>Immunoassay</subject><subject>Immunoassays</subject><subject>Infectious diseases</subject><subject>Microbiology</subject><subject>Milk</subject><subject>Multiplexing</subject><subject>Mycotoxins</subject><subject>Ochratoxin A</subject><subject>Proteins</subject><subject>Residues</subject><issn>1936-9751</issn><issn>1936-976X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9UF1LwzAUDaLgnP4BnwI-R5M0TdtHGeoGG4JO8C1kadpla5uatLj-e6P1480Ll3sezgf3AHBJ8DXBOLnxhBJOECZpWEYoYkdgQrKIoyzhr8e_OCan4Mz7HcYcB9oE7Fd91Zm20gd4X9l3OBs6W-vOGQUXdd03VnovBw8L6-DclFu03jrbl9u27-Czclo3pimhLeCPD1wNynb2YBr4pL3Je-1hwCtT7c_BSSErry--7xS83N-tZ3O0fHxYzG6XSEU86hBLUsIUl1pRJnONOc-SPGGMh8kI43ojFVcqipM8_CspZ3hDlVYFjnXGUxVNwdXo2zr7FvI7sbO9a0KkoCRLeUTjhAYWHVnKWe-dLkTrTC3dIAgWn6WKsVQRQsRXqYIFUTSKfCA3pXZ_1v-oPgCQEnta</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Qu, Jianwen</creator><creator>Xie, Huijuan</creator><creator>Zhang, Shuying</creator><creator>Luo, Pengjie</creator><creator>Guo, Ping</creator><creator>Chen, Xianxiong</creator><creator>Ke, Yuebin</creator><creator>Zhuang, Junyu</creator><creator>Zhou, Fangmei</creator><creator>Jiang, Wenxiao</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-2443-7648</orcidid></search><sort><creationdate>20190401</creationdate><title>Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk</title><author>Qu, Jianwen ; Xie, Huijuan ; Zhang, Shuying ; Luo, Pengjie ; Guo, Ping ; Chen, Xianxiong ; Ke, Yuebin ; Zhuang, Junyu ; Zhou, Fangmei ; Jiang, Wenxiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-47814c6aec24ade06697d74466669146ebac6cc357d018a2640b2cecf05e968c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aflatoxin M1</topic><topic>Aflatoxins</topic><topic>Analytical Chemistry</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Deoxynivalenol</topic><topic>Detection limits</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Flow cytometry</topic><topic>Food Science</topic><topic>Fumonisin B1</topic><topic>High-throughput screening</topic><topic>Immunoassay</topic><topic>Immunoassays</topic><topic>Infectious diseases</topic><topic>Microbiology</topic><topic>Milk</topic><topic>Multiplexing</topic><topic>Mycotoxins</topic><topic>Ochratoxin A</topic><topic>Proteins</topic><topic>Residues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qu, Jianwen</creatorcontrib><creatorcontrib>Xie, Huijuan</creatorcontrib><creatorcontrib>Zhang, Shuying</creatorcontrib><creatorcontrib>Luo, Pengjie</creatorcontrib><creatorcontrib>Guo, Ping</creatorcontrib><creatorcontrib>Chen, Xianxiong</creatorcontrib><creatorcontrib>Ke, Yuebin</creatorcontrib><creatorcontrib>Zhuang, Junyu</creatorcontrib><creatorcontrib>Zhou, Fangmei</creatorcontrib><creatorcontrib>Jiang, Wenxiao</creatorcontrib><collection>CrossRef</collection><jtitle>Food analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qu, Jianwen</au><au>Xie, Huijuan</au><au>Zhang, Shuying</au><au>Luo, Pengjie</au><au>Guo, Ping</au><au>Chen, Xianxiong</au><au>Ke, Yuebin</au><au>Zhuang, Junyu</au><au>Zhou, Fangmei</au><au>Jiang, Wenxiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk</atitle><jtitle>Food analytical methods</jtitle><stitle>Food Anal. Methods</stitle><date>2019-04-01</date><risdate>2019</risdate><volume>12</volume><issue>4</issue><spage>877</spage><epage>886</epage><pages>877-886</pages><issn>1936-9751</issn><eissn>1936-976X</eissn><abstract>Microsphere-based immunoassays involving flow cytometry have recently gained popularity for use in protein detection and infectious disease diagnosis due to their simple assay format and capacity for multiplexed analysis. In this work, four mycotoxins, including aflatoxin M
1
(AFM
1
), ochratoxin A (OTA), deoxynivalenol (DON), and fumonisin B
1
(FB
1
), were selected as model analytes. A quadplex flow cytometric immunoassay (FCIA) was developed for detecting multiple mycotoxin residues in milk. The optimized quadplex FCIA exhibited satisfactory compatibility with the multiple mycotoxin residue analysis, with limits of detection (LODs) of 0.045 μg L
−1
for AFM
1
, 0.94 μg L
−1
for OTA, 7.48 μg L
−1
for DON, and 2.45 μg L
−1
for FB
1
. The recoveries of the target mycotoxins from spiked milk were 76–95%, with a relative standard deviation of less than 13.4%. Compared to traditional ELISA formats, the FCIA provided lower detection limits for multiple mycotoxin detection.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12161-018-01412-4</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-2443-7648</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1936-9751 |
| ispartof | Food analytical methods, 2019-04, Vol.12 (4), p.877-886 |
| issn | 1936-9751 1936-976X |
| language | eng |
| recordid | cdi_proquest_journals_2198632572 |
| source | Springer Nature |
| subjects | Aflatoxin M1 Aflatoxins Analytical Chemistry Chemistry Chemistry and Materials Science Chemistry/Food Science Deoxynivalenol Detection limits Enzyme-linked immunosorbent assay Flow cytometry Food Science Fumonisin B1 High-throughput screening Immunoassay Immunoassays Infectious diseases Microbiology Milk Multiplexing Mycotoxins Ochratoxin A Proteins Residues |
| title | Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk |
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