Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

• Published in: Sensors and actuators. B, Chemical Chemical, 2019-04, Vol.285, p.601-606
• Main Authors: Viter, Roman, Savchuk, Maryna, Starodub, Nickolay, Balevicius, Zigmas, Tumenas, Saulius, Ramanaviciene, Almira, Jevdokimovs, Daniels, Erts, Donats, Iatsunskyi, Igor, Ramanavicius, Arunas
• Format: Article
• Language: English
• Subjects: Antibodies; Antigen-antibody complex; Antigens; Biomolecules; Bovine leukemia virus (BLV); Glass; Immobilization; Immunosensors; Leukemia; Nanorods; Optical immunosensor; Photoluminescence; Polyallylamine hydrochloride; Proteins; Selectivity; Serum albumin; Viruses; Zinc oxide; ZnO nanorods
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2019.01.054

[Display omitted] •Bovine leukaemia virus (BLV) proteins gp51 were in immunosensor design as bio-receptor.•Immobilization efficiency of gp51 on glass surface modified by ZnO nanorod (ZnO-NR) was evaluated.•The applicability of gp51/ZnO-NR/glass in the design of photoluminescence based immunosensor was tested.•The formation of antigen-antibody complex on the gp51/ZnO-NR/glass modified was investigated.•Antibodies against BLV proteins (anti-gp51) were detected in blood serum of BLV infected cattle. Bovine leukaemia virus (BLV) proteins gp51, which are serving as antigens for specific antibodies against BLV proteins (anti-gp51), were applied as biological recognition part in the design of immunosensor devoted for the determination of anti-gp51. The efficiency of the immobilization of BLV proteins gp51 on ZnO nanorod (ZnO-NR) modified glass (ZnO-NR/glass) surface was evaluated. The formation of antigen-antibody complex on the ZnO/glass modified by the BLV proteins gp51 (gp51/ZnO-NR/glass) was investigated by the determination of changes in ZnO photoluminescence. The applicability of gp51/ZnO-NR/glass in the design of photoluminescence based immunosensor was evaluated. Bovine serum albumin (BSA) was applied for the modification of sensing gp51 layer in order to form gp51&BSA layer with advanced selectivity. Polyallylamine hydrochloride (PAH) was applied in order to improve the immobilization of gp51 and BSA based sensing layer (gp51&BSA) on the surface of ZnO-NR/glass. PAH was applied during the formation of gp51&BSA/PAH/ZnO-NR/glass structure. Some aspects of the mechanism of interaction between biomolecules (gp51, BSA and anti-gp51) and ZnO-NR during the preparation and action of gp51&BSA/ZnO-NR/glass- and gp51&BSA/PAH/ZnO-NR/glass-based immunosensors have been discussed.