Identification of urine PLK2 as a marker of bladder tumors by proteomic analysis

Objective To determine the patterns of urinary proteins in bladder cancer subjects using pooled urine from 27 bladder cancer patients. Methods The urine matrix was removed by acetonitrile precipitation followed by molecular weight cutoff. High performance liquid chromatography coupled with electrosp...

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Bibliographic Details
Published in:World journal of urology 2010-02, Vol.28 (1), p.117-122
Main Authors: Tan, Lia-Beng, Chen, Kow-Tong, Yuan, Yu-Chang, Liao, Pao-Chi, Guo, How-Ran
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Biomarkers, Tumor - genetics
Biomarkers, Tumor - urine
Female
Humans
Male
Medical sciences
Medicine
Medicine & Public Health
Middle Aged
Nephrology
Nephrology. Urinary tract diseases
Oncology
Original Article
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - urine
Proteomics
Tumors of the urinary system
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - urine
Urinary system involvement in other diseases. Miscellaneous
Urinary tract. Prostate gland
Urology
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Summary:Objective To determine the patterns of urinary proteins in bladder cancer subjects using pooled urine from 27 bladder cancer patients. Methods The urine matrix was removed by acetonitrile precipitation followed by molecular weight cutoff. High performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC–ESI-MS/MS) was used to analyze the urinary proteome. The candidate protein was validated by western blot. Results A total of 146 urinary proteins were identified. The protein list was screened using bioinformatic tools, and 11 cancer-related urinary proteins were found to be potential tumor markers for bladder cancer. However, only PLK2 was identified with high confidence. This candidate protein was validated by western blot using urine samples from 14 normal subjects and 10 bladder cancer patients. Statistically significant correlations were detected between PLK2 expression and bladder transitional cell carcinoma (TCC) ( P  
ISSN:0724-4983
1433-8726
DOI:10.1007/s00345-009-0432-y