Method validation and simultaneous quantification of eight organochlorines/organophosphates in apple by gas chromatography

BACKGROUND The increasing and extensive use of pesticides worldwide has resulted in a significant loss of non‐target populations particularly humans by direct or indirect exposures. Also, various methods have been used for the estimation of pesticide residues in fruits and vegetables from recent pas...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2019-05, Vol.99 (7), p.3687-3692
Main Authors: Wani, Ashraf A, Dar, Alamgir A, Jan, Ishrat, Sofi, Khurshid A, Sofi, Javid A, Dar, Irshad H
Format: Article
Language:English
Subjects:
Apples
Calibration
Chlorpyrifos
Chromatography
Chromatography, Gas - methods
Cypermethrin
Fruit - chemistry
Fruits
Gas chromatography
Hydrocarbons, Chlorinated - analysis
Insecticides
Limit of Detection
Linearity
Malus - chemistry
method validation
Organic chemistry
Organochlorine compounds
organochlorines
Organophosphates
Organophosphates - analysis
Organothiophosphates - analysis
Pesticide residues
Pesticide Residues - analysis
Pesticides
Pyrethrins - analysis
Residues
Standardization
Vegetables
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Summary:BACKGROUND The increasing and extensive use of pesticides worldwide has resulted in a significant loss of non‐target populations particularly humans by direct or indirect exposures. Also, various methods have been used for the estimation of pesticide residues in fruits and vegetables from recent past which are either tedious, time consuming or expensive. Therefore, the present study was performed to determine the pesticide residues from apple by simple and novel validated gas chromatography. RESULTS A novel, accurate, ecofriendly and cost‐effective gas chromatography method was developed for simultaneous quantification of eight pesticides, namely chlorpyrifos‐methyl (1), chlorpyrifos (2), quinolphos (3), profenofos (4), myclobutnil (5), ethion (6), fenpropathrin (7) and cypermethrin (8). The developed method was validated as per the SANTE guidelines. All calibration curves showed a good linear relationship (r > 0.99) within the test range. Precision was evaluated by intra‐ and inter‐day tests with relative standard deviations (RSDs) < 2.0%, recovery in between 70% and 120% with RSDs < 2.00%. CONCLUSION The results demonstrate that the concentration of pesticides 1 to 8 were found below the detectable limit. Method validation parameters like linearity, precision, accuracy, specificity, robustness, detection and quantification limits were found within the acceptable range. The proposed method makes it possible to determine simultaneously pesticides 1–8 in one run which can be extended for residue‐based standardization of pesticides from apple and other fruits and vegetables. © 2019 Society of Chemical Industry
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.9599