Survival and internalization of Salmonella and Escherichia coli O157:H7 sprayed onto different cabbage cultivars during cultivation in growth chambers

• Published in: Journal of the science of food and agriculture 2019-05, Vol.99 (7), p.3530-3537
• Main Authors: Erickson, Marilyn C, Liao, Jye‐Yin, Payton, Alison S, Cook, Peter W, Ortega, Ynes R
• Format: Article
• Language: English
• Subjects: Agricultural pollution; Antiinfectives and antibacterials; antioxidant capacity; Bacteria; Brassica - growth & development; Brassica - microbiology; Brassica oleracea; Colony Count, Microbial; Contamination; Cultivars; Cultivation; E coli; Escherichia coli; Escherichia coli O157 - growth & development; Food Contamination - analysis; Growth chambers; Internalization; Microbial contamination; Microbial Viability; Organic chemistry; pathogen; Pathogens; phenols; pre‐harvest; Risk reduction; Salmonella; Salmonella - growth & development; Survival
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.9573

BACKGROUND Cabbage may become contaminated with enteric pathogens during cultivation. Using multiple cabbage cultivars at two maturity stages (small plants or plants with small heads) in growth chamber studies, the fate (internalization or surface survival) of Salmonella and Escherichia coli O157:H7 (0157) were examined in conjunction with any potential relationships to the plant's antimicrobial content. RESULTS Internalized Salmonella was detected in cabbage within 24 h with prevalence ranging from 62% (16 of 26) for the ‘Super Red 80’ cultivar to 92% (24 of 26) for the ‘Red Dynasty’ cultivar. Surface survival of pathogens on small cabbage plants over nine days was significantly affected by cultivar with both pathogens surviving the most on the ‘Farao’ cultivar and Salmonella and O157 surviving the least on the ‘Super Red 80’ and ‘Capture’ cultivars, respectively (P < 0.05). Survival of O157 was slightly higher on cabbage heads for O157 than small plants suggesting that the maturity stage may affect this pathogen's fate. An inverse relationship existed between antimicrobial levels and the pathogen's surface survival on cabbage heads (P < 0.05). CONCLUSIONS The fate of pathogens varied with the cabbage cultivar in growth chamber studies highlighting the potential to explore cultivar in field studies to reduce the risk of microbiological contamination in this crop. © 2019 Society of Chemical Industry