Overexpression of myocyte enhancer factor 2 and histone hyperacetylation in hepatocellular carcinoma

Purpose It has been indicated that activated hepatic stellate cells (HSCs) play key roles on the pathogenesis of hepatocellular carcinoma (HCC). The purpose of the study was to investigate the potential mechanism in it. Methods Activation of HSCs, the expression of myocyte enhancer factor 2 (MEF2),...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 2008-01, Vol.134 (1), p.83-91
Main Authors: Bai, XueLi, Wu, LiHua, Liang, TingBo, Liu, ZhiQiang, Li, JunJian, Li, DongLin, Xie, HaiYang, Yin, ShengYong, Yu, Jun, Lin, Qi, Zheng, ShuSen
Format: Article
Language:English
Subjects:
Acetylation - drug effects
Antineoplastic agents
Biological and medical sciences
Blotting, Western
Cancer
Cancer Research
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Carcinoma, Hepatocellular - therapy
Gastroenterology. Liver. Pancreas. Abdomen
Gene expression
Hematology
Histone Deacetylases - genetics
Histone Deacetylases - metabolism
Histones - metabolism
Humans
Immunoenzyme Techniques
Internal Medicine
Liver - cytology
Liver - metabolism
Liver Cirrhosis - metabolism
Liver Cirrhosis - pathology
Liver Cirrhosis - therapy
Liver diseases
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
MADS Domain Proteins - genetics
MADS Domain Proteins - metabolism
Medical sciences
Medicine
Medicine & Public Health
MEF2 Transcription Factors
Myogenic Regulatory Factors - genetics
Myogenic Regulatory Factors - metabolism
Oncology
Original Paper
Pathology
Pharmacology. Drug treatments
Proteins
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Tissue Array Analysis
Tumors
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Summary:Purpose It has been indicated that activated hepatic stellate cells (HSCs) play key roles on the pathogenesis of hepatocellular carcinoma (HCC). The purpose of the study was to investigate the potential mechanism in it. Methods Activation of HSCs, the expression of myocyte enhancer factor 2 (MEF2), class II histone deacetylases (II HDACs) and histone acetylation were analyzed in specimens of primary HCCs, cirrhotic and normal livers. Activated HSCs were identified using anti-a-smooth muscle actin (a-SMA) by Immunohistochemistry (IHC). The levels of expression of MEF2A, MEF2C and II HDACs mRNA and protein were measured by real time quantitative PCR and western blot (WB). Histone acetylation was assessed using anti-acetyl-histone H3, -H4 by WB and IHC. A P value 
ISSN:0171-5216
1432-1335
DOI:10.1007/s00432-007-0252-7