Knockdown of sal‐like 4 expression by small interfering RNA induces apoptosis in breast cancer cells

Breast cancer is the most prevalent cancers worldwide and causes a significant amount of deaths annually. Spalt‐like transcription factor 4 is known as a transcription factor, which has an important role in the proliferation of cancerous cells. Small interfering RNA (siRNA) is a short‐chain molecule...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2019-06, Vol.120 (6), p.9392-9399
Main Authors: Hesari, Amireza, Anoshiravani, Ali Arash, Talebi, Samaneh, Noruzi, Somayye, Mohammadi, Rezvan, Salarinia, Reza, Zare, Reza, Ghasemi, Faezeh
Format: Article
Language:English
Subjects:
Apoptosis
biomarker
Breast cancer
Cancer
Cell proliferation
Chemotherapy
diagnosis
Gene expression
Molecular chains
Nucleotide sequence
Nucleotides
Polymerase chain reaction
Ribonucleic acid
RNA
Side effects
siRNA
spalt‐like transcription factor 4
Transcription factors
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Summary:Breast cancer is the most prevalent cancers worldwide and causes a significant amount of deaths annually. Spalt‐like transcription factor 4 is known as a transcription factor, which has an important role in the proliferation of cancerous cells. Small interfering RNA (siRNA) is a short‐chain molecule of 20 to 25 nucleotides that protrude on two sides of the 3′, two nucleotides. In this study, using a specific sequence of siRNA against the sequence of this gene, its activity is investigated in the cell line of breast cancer. The breast cancer cells (MCF‐7) were cultured and then, using a specific anti‐sal–like 4 (SALL4) siRNA, their toxic doses were determined. Then, the gene is transfected into the cell. Proliferation and expression of the SALL4 and BCL‐2 gene were measured using the real‐time polymerase chain reaction method. The specific concentration of siRNA IC50 of the SALL4 gene was 40.35 nmole. Gene expression results indicated that the expression of the Bcl‐2 gene in the siRNA group was significantly reduced ( P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.28214