Lactic Acid Production from a Whole Slurry of Acid-Pretreated Spent Coffee Grounds by Engineered Saccharomyces cerevisiae

Spent coffee grounds (SCG) generated after coffee extraction are the main byproduct of the coffee industry. Valorization of the SCG has been increasingly focused following considerable attention in coffee consumption. Lactic acid bacteria fermentation is the primary source of generation of lactic ac...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2019-09, Vol.189 (1), p.206-216
Main Authors: Kim, Jeong-won, Jang, Jeong Hwa, Yeo, Hyeon Jin, Seol, Jeongman, Kim, Soo Rin, Jung, Young Hoon
Format: Article
Language:English
Subjects:
Acid production
Bacteria
Baking yeast
Biochemistry
Biotechnology
Chemistry
Chemistry and Materials Science
Coffee
Ethanol
Feasibility studies
Fermentation
Hemicellulose
Industrial applications
L-Lactate dehydrogenase
Lactate dehydrogenase
Lactic acid
Lactic acid bacteria
Polylactic acid
Saccharification
Saccharomyces cerevisiae
Slurries
Substrates
Xylose
Yeast
Yeasts
Yogurt
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Summary:Spent coffee grounds (SCG) generated after coffee extraction are the main byproduct of the coffee industry. Valorization of the SCG has been increasingly focused following considerable attention in coffee consumption. Lactic acid bacteria fermentation is the primary source of generation of lactic acid, a monomer of polylactic acid that has various industrial applications; however, because of the low tolerance of lactic acid bacteria to toxic compounds, it is necessary to apply Saccharomyces cerevisiae to produce lactic acid whose tolerance to toxic compounds is higher. In this study, we evaluated the feasibility of using SCG as substrate for the production of lactic acid by S. cerevisiae strain expressing heterologous lactate dehydrogenase. The fermentation profiles of the engineered yeast showed that lactic acid production was promoted by xylose addition. From simultaneous saccharification and fermentation (SSF) using a whole slurry of acid-pretreated SCG, containing high amounts of hemicellulose fractions, lactic acid (0.11 g) and ethanol (0.10 g) per g SCG were obtained after 24 h of SSF, of which yields were 413% and 221% higher, respectively, than those of washed pretreated SCG. Thus, fermentation of whole slurry SCG by engineered S. cerevisiae is a suitable way of lactic acid production, selectively.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-019-03000-6