Molecular detection and phylogenetic tree analysis of Salmonella Pullorum isolated from broiler chicken in Al-Diwanyia province, Iraq

When studied the phylogeny, the confirmed isolates (No.1 (MH752058), No.3 (MH752060), and No.4 (MH752061)) showed 99% similarity to the NCBI isolate, MH421997.1, an isolate from Colombia. [...]the current study isolate No.2, MH752059, revealed 99% matching to the NCBI isolate, MH446374.1, another is...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical sciences and research 2019-03, Vol.11 (3), p.759-761
Main Authors: Atyia, Hiba Turkey, Al-Mahmoudi, Abbas Hadi Jasim, Sadeq, Jenan Nadhim
Format: Article
Language:English
Subjects:
Anorexia
Antigens
Deoxyribonucleic acid
DNA
Infections
Internet
Phylogenetics
Poultry
Salmonella
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:When studied the phylogeny, the confirmed isolates (No.1 (MH752058), No.3 (MH752060), and No.4 (MH752061)) showed 99% similarity to the NCBI isolate, MH421997.1, an isolate from Colombia. [...]the current study isolate No.2, MH752059, revealed 99% matching to the NCBI isolate, MH446374.1, another isolate from Colombia. The disease is characterized by the presence of certain clinical features such as anorexia, diarrhea, and high mortality in chicks and declines in egg production and reproduction-based features such as fertility and hatchability and high mortalities in adult birds. PCR method The technique used in this study utilized the 16S rRNA gene as a main target to detect Salmonella Pullorum using specific primers, GenBank: FR686362.1, that were designed using the NCBI-based website and Primer 3 Plus online and purchased from (Bioneer, Korea). The products of the PCR test were run on a agarose gel at 1% contained the dye of ethidium bromide and electrophoresed to be later visualized under a UV imager. When studied the phylogeny, the confirmed isolates (No.1 (MH752058), No.3 (MH752060), and No.4 (MH752061)) showed 99% similarity to the NCBI isolate, MH421997.1, an isolate from Colombia. [...]the current study isolate No.2, MH752059, revealed 99% matching to the NCBI isolate, MH446374.1, another isolate from Colombia. The disease is characterized by the presence of certain clinical features such as anorexia, diarrhea, and high mortality in chicks and declines in egg production and reproduction-based features such as fertility and hatchability and high mortalities in adult birds (2).
ISSN:0975-1459