Analysis of virulence factors and emm typing of Streptococcus pyogenes clinical isolates

Background: Streptococcus pyogenes, a Group A streptococci (GAS), is an important human pathogen that causes a wide range of infections. Methods: Twenty five clinical isolates of S. pyogenes were submitted to an emm typing and to a Real-time PCR analysis for 23 important virulence factors. Results:...

Full description

Saved in:
Bibliographic Details
Published in:PeerJ preprints 2019-04
Main Authors: Gustavo Enck Sambrano, Riboldi, Gustavo P, Reiter, Keli C, Thiago Galvão da Silva Paim, Neidmar Correa Tolfo, Soares, Renata O, d'Azevedo, Pedro A
Format: Article
Language:English
Subjects:
Clinical isolates
Pathogens
Peptidase
Strains (organisms)
Streptococcus pyogenes
Tropism
Typing
Virulence factors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Streptococcus pyogenes, a Group A streptococci (GAS), is an important human pathogen that causes a wide range of infections. Methods: Twenty five clinical isolates of S. pyogenes were submitted to an emm typing and to a Real-time PCR analysis for 23 important virulence factors. Results: Fourteen emm types were found and the emm1 type was the most prevalent. The majority of the isolates were classified as emm pattern E, followed by A-C3. No pattern D was found. Among the virulence factors, the most prevalent were SpeG, Slo, C5a-peptidase and SPNA. Phage encoded virulence genes were also found among the strains, such as mf-2, SpeJ and SpeL. Discussion: The emm1 type was the most prevalent while the 13 others M types were distributed along the strains. No tissue tropism was found on the isolates. The virulence factors analysis demonstrated that chromosomally and phage-encoded genes were found, which confers a potential for high virulent micro-organisms.
ISSN:2167-9843
DOI:10.7287/peerj.preprints.27649v1