Cytotoxicity of the phytosterol diosgenin and its derivatives in rat cultured hepatocytes and V79 fibroblasts

In this work, the cytotoxic effects of some spirostane derivatives were examined in cultured hepatocytes and V79 fibroblasts using different viability assays. The derivatives were obtained by modifying the A and B rings of diosgenin. Diosgenin and its derivatives were more toxic in V79 fibroblasts (...

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Bibliographic Details
Published in:Human & experimental toxicology 2004-10, Vol.23 (10), p.487-493
Main Authors: Melo, Patricia Silva, De Azevedo, Mariangela Burgos Martins, Zullo, Marco Antônio Teixeira, Fabrin-Neto, João Batista, Haun, Marcela
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Count
Cell Line
Cell Survival - drug effects
Cimetidine - pharmacology
Cricetinae
Cricetulus
Cytotoxicity
Diosgenin - analogs & derivatives
Diosgenin - toxicity
DNA - analysis
Dose-Response Relationship, Drug
Drug Combinations
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - metabolism
Fibroblasts - pathology
Hepatocytes - drug effects
Hepatocytes - metabolism
Hepatocytes - pathology
Medical sciences
Metabolism
Neutral Red - metabolism
Penicillin
Plant poisons toxicology
Rats
Tetrazolium Salts - metabolism
Toxicity
Toxicology
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Summary:In this work, the cytotoxic effects of some spirostane derivatives were examined in cultured hepatocytes and V79 fibroblasts using different viability assays. The derivatives were obtained by modifying the A and B rings of diosgenin. Diosgenin and its derivatives were more toxic in V79 fibroblasts (IC50 40-300 µM) than in hepatocytes (IC50 280-1000 µM). Inhibition of cytochrome P450IIIA in cultured hepatocytes by incubation with 1 mM cimetidine did not alter the toxicity of these compounds in these cells. These observations suggest that other pathways of detoxification may be involved in hepatocytes. In conclusion, the compounds studied merit investigation for their potential pharmacological and industrial applicability.
ISSN:0960-3271
1477-0903
DOI:10.1191/0960327104ht474oa