Huygens STED Deconvolution Increases Signal-to-Noise and Image Resolution towards 22 nm

Image restoration aims to make optimal use of the data provided by the light microscope to recover the object being imaged; in other words, to “figure out what the microscope is actually trying to tell us.” To achieve this, the distortions introduced by the imaging process need to be undone insofar...

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Bibliographic Details
Published in:Microscopy today 2013-11, Vol.21 (6), p.38-44
Main Authors: Schoonderwoert, Vincent, Dijkstra, Remko, Luckinavicius, Grazvydas, Kobler, Oliver, van der Voort, Hans
Format: Magazinearticle
Language:English
Subjects:
Image Processing
Image resolution
Image restoration
Lasers
Microscopy
Noise
Optics
Researchers
Success
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Summary:Image restoration aims to make optimal use of the data provided by the light microscope to recover the object being imaged; in other words, to “figure out what the microscope is actually trying to tell us.” To achieve this, the distortions introduced by the imaging process need to be undone insofar possible.
ISSN:1551-9295
2150-3583
DOI:10.1017/S1551929513001089