Restoration of Light Sheet Multi-View Data with the Huygens Fusion and Deconvolution Wizard

Light sheet fluorescence microscopy (LSFM) allows for high-resolution three-dimensional imaging with minimal photo-damage. By viewing the sample from different directions, different regions of large specimens can be imaged optimally. Moreover, owing to their good spatial resolution and high signal-t...

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Bibliographic Details
Published in:Microscopy today 2018-09, Vol.26 (5), p.12-19
Main Authors: Verveer, Peter J., Schoonderwoert, Vincent T.G., Ressnikoff, Denis, Elliott, Shane D., van Teutem, Kiefer D., Walther, Tobias C., van der Voort, Hans T.M.
Format: Magazinearticle
Language:English
Subjects:
Algorithms
Deconvolution
Developmental biology
Fluorescence
Image resolution
Image restoration
Light
Light Microscopy
Microscopy
Noise
Optics
Spatial resolution
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Summary:Light sheet fluorescence microscopy (LSFM) allows for high-resolution three-dimensional imaging with minimal photo-damage. By viewing the sample from different directions, different regions of large specimens can be imaged optimally. Moreover, owing to their good spatial resolution and high signal-to-noise ratio, LSFM data are well suited for image deconvolution. Here we present the Huygens Fusion and Deconvolution Wizard, a unique integrated solution for restoring LSFM images, and show that improvements in signal and resolution of 1.5 times and higher are feasible.
ISSN:1551-9295
2150-3583
DOI:10.1017/S1551929518000846