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Restoration of Light Sheet Multi-View Data with the Huygens Fusion and Deconvolution Wizard

Light sheet fluorescence microscopy (LSFM) allows for high-resolution three-dimensional imaging with minimal photo-damage. By viewing the sample from different directions, different regions of large specimens can be imaged optimally. Moreover, owing to their good spatial resolution and high signal-t...

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Published in:	Microscopy today 2018-09, Vol.26 (5), p.12-19
Main Authors:	Verveer, Peter J., Schoonderwoert, Vincent T.G., Ressnikoff, Denis, Elliott, Shane D., van Teutem, Kiefer D., Walther, Tobias C., van der Voort, Hans T.M.
Format:	Magazinearticle
Language:	English
Subjects:	Algorithms Deconvolution Developmental biology Fluorescence Image resolution Image restoration Light Light Microscopy Microscopy Noise Optics Spatial resolution
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description	Light sheet fluorescence microscopy (LSFM) allows for high-resolution three-dimensional imaging with minimal photo-damage. By viewing the sample from different directions, different regions of large specimens can be imaged optimally. Moreover, owing to their good spatial resolution and high signal-to-noise ratio, LSFM data are well suited for image deconvolution. Here we present the Huygens Fusion and Deconvolution Wizard, a unique integrated solution for restoring LSFM images, and show that improvements in signal and resolution of 1.5 times and higher are feasible.
doi_str_mv	10.1017/S1551929518000846
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source	Oxford Journals Online; EZB Electronic Journals Library
subjects	Algorithms Deconvolution Developmental biology Fluorescence Image resolution Image restoration Light Light Microscopy Microscopy Noise Optics Spatial resolution
title	Restoration of Light Sheet Multi-View Data with the Huygens Fusion and Deconvolution Wizard
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