Multidrug-resistant MCF-7 cells: An identity crisis? / Response

MCF-7 cells express a truncated isoform of caspase-3 transcript, whereas drug-resistant sublines express fulllength caspase-3 transcript. Thus, we amplified the complementary DNA (cDNA) from MCF-7 and MCF-7/WT/ DOX cells by polymerase chain reaction (PCR) using primers specific for fulllength caspas...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 2002-11, Vol.94 (21), p.1652
Main Authors: Mehta, Kapil, Devarajan, Eswaran, Chen, Jack, Multani, Asha
Format: Article
Language:English
Subjects:
Cells
Drug resistance
Online Access:Get full text
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Summary:MCF-7 cells express a truncated isoform of caspase-3 transcript, whereas drug-resistant sublines express fulllength caspase-3 transcript. Thus, we amplified the complementary DNA (cDNA) from MCF-7 and MCF-7/WT/ DOX cells by polymerase chain reaction (PCR) using primers specific for fulllength caspase-3 cDNA to further validate the presence of a drug-resistant clone in the parental MCF-7 cells.
ISSN:0027-8874
1460-2105