2DE-DIGE proteomic study in blood plasma of patients in childhood stage with acute lymphoblastic leukemia

In Colombia, during the last decade, acute lymphoblastic leukemia (ALL) has caused more than 40% of cancer deaths in children. Among the factors that influence these figures, late diagnosis is one of the factors that affects the treatment success. Therefore, this research focused on the plasma prote...

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Bibliographic Details
Published in:Revista colombiana de química 2019-01, Vol.48 (1), p.5
Main Authors: Yadi Adriana Umaña-Pérez, Calderón Rodriguez, Sandra Isabel
Format: Article
Language:Spanish
Subjects:
Biomarkers
Blood plasma
Cancer
Diagnostic systems
Glycoproteins
Leukemia
Mass spectrometry
Patients
Proteins
Proteomics
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Summary:In Colombia, during the last decade, acute lymphoblastic leukemia (ALL) has caused more than 40% of cancer deaths in children. Among the factors that influence these figures, late diagnosis is one of the factors that affects the treatment success. Therefore, this research focused on the plasma proteome study of Colombian children diagnosed with B-cell ALL, as compared with healthy controls in the search of proteins that could be classified as diagnostic biomarkers. Now, in view of the advances in the proteomics and mass spectrometry tools and taking into account that they are an alternative to address the molecular complexity of diseases such as cancer, a proteomic approach, based on bidimensional difference gel electrophoretic separation (2DE-DIGE) coupled to LC-MS/MS, was used. We found eight differentially expressed proteins in plasma from B-cell ALL patients as follows: Serotransferrin, Alpha-1-antitrypsin, Haptoglobin, Zinc-alpha-2-glycoprotein, and Complement C3.
ISSN:0120-2804
2357-3791
DOI:10.15446/rev.colomb.quim.v48n1.75170