ANTI QUORUM SENSING AND ANTI BIOFILM POTENTIAL OF ANOGEISSUS ACUMINATA AND MALLOTUS ROXBURGHIANUS MUELL. AGAINST PSEUDOMONAS AERUGINOSA PAO1

Pseudomonas aeruginosa causes severe persistent infections in immunocompromised patients by showing tolerance conventional antibiotic therapy by virtue of specialized hierarchy of quorum sensing (QS) network. The purpose of the study was to evaluate the efficacy of two Mizoram medicinal plants i.e.,...

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Bibliographic Details
Published in:Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2019-04, Vol.8 (5), p.1135-1140
Main Authors: Hnamte, Sairengpuii, Subhaswaraj, Pattnaik, Kumar Ranganathan, Sampath, Rao Ampasala, Dinakara, Muralitharan, Gangatharan, Siddhardha, Busi
Format: Article
Language:English
Subjects:
Alginates
Alginic acid
Antibiotics
Binding
Biofilms
Effectiveness
Elastase
Exopolysaccharides
Gas chromatography
Herbal medicine
Immunocompromised hosts
Mass spectrometry
Mass spectroscopy
Medicinal plants
Pathogens
Phenotypes
Phytochemicals
Plant extracts
Proteins
Pseudomonas aeruginosa
Pyocyanin
Quorum sensing
Virulence
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Summary:Pseudomonas aeruginosa causes severe persistent infections in immunocompromised patients by showing tolerance conventional antibiotic therapy by virtue of specialized hierarchy of quorum sensing (QS) network. The purpose of the study was to evaluate the efficacy of two Mizoram medicinal plants i.e., Anogeissus acuminata and Mallotus roxburghianus Muell. in attenuating QS regulated P. aeruginosa virulence. The crude leaf extracts were tested for their ability to combat QS associated virulence phenotypes such as pyocyanin production, LasB elastase and Staphylolytic activity and production of biofilm determinants such as alginate and exopolysaccharide in P. aeruginosa PAO1. The gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of phytocompounds, the synergistic activity of which is responsible for the anti QS activity. The in silico studies provide the binding efficacy of obtained phytochemical with QS receptor protein, LasR. The in vitro studies followed by in silico analysis demonstrated the efficacy of phytochemical of A. acuminata and M. roxburghianus Muell. in competitively inhibiting the binding of natural ligand with LasR and thereby altering production of virulence phenotypes. The efficacy in down regulating bacterial virulence shown by A. acuminata and M. roxburghianus Muell. provides promising alternatives to develop next generation anti-pathogenic agents.
ISSN:1338-5178
1338-5178
DOI:10.15414/jmbfs.2019.8.5.1135-1140